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Western Blot (WB) (Western Blot: Sample: Recombinant ARO, Rat)

Rabbit anti-Rat Aromatase (ARO) Polyclonal Antibody | anti-ARO antibody

Polyclonal Antibody to Aromatase (ARO)

Gene Names
Cyp19a1; Cyp19; Cyp19a; p450arom; Aromatase
Reactivity
Rat
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunoprecipitation
Purity
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Synonyms
Aromatase (ARO); Polyclonal Antibody; Polyclonal Antibody to Aromatase (ARO); CYP19A1; CYPXIX; ARO; ARO1; CPV1; CYAR; CYP19; P450AROM; P450-AROM; Estrogen synthase; Cytochrome P450; Family 19; Subfamily A; Polypeptide 1; anti-ARO antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Rat
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against ARO. It has been selected for its ability to recognize ARO in immunohistochemical staining and western blotting.
Purity/Purification
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Form/Format
Liquid
Concentration
500ug/mL (varies by lot)
Sequence Length
508
Applicable Applications for anti-ARO antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)
Application Notes
Western blotting: 0.2-2ug/mL;1:250-2500
Immunohistochemistry: 5-20ug/mL;1:25-100
Immunocytochemistry: 5-20ug/mL;1:25-100
Optimal working dilutions must be determined by end user.
Immunogen
Recombinant Aromatase (ARO)
Cross Reactivity
Rat
Buffer Formulation
0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Western Blot (WB)

(Western Blot: Sample: Recombinant ARO, Rat)

Western Blot (WB) (Western Blot: Sample: Recombinant ARO, Rat)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
58,412 Da
NCBI Official Full Name
Aromatase
NCBI Official Synonym Full Names
cytochrome P450, family 19, subfamily a, polypeptide 1
NCBI Official Symbol
Cyp19a1
NCBI Official Synonym Symbols
Cyp19; Cyp19a; p450arom; Aromatase
NCBI Protein Information
aromatase
UniProt Protein Name
Aromatase
UniProt Gene Name
Cyp19a1
UniProt Synonym Gene Names
Arom; Cyp19

NCBI Description

catalyzes end-step estrogen formation from androgens and aromatization of testosterone to estradiol [RGD, Feb 2006]

Uniprot Description

CYP19A1: Catalyzes the formation of aromatic C18 estrogens from C19 androgens. Belongs to the cytochrome P450 family.

Protein type: EC 1.14.14.14; Lipid Metabolism - androgen and estrogen; Oxidoreductase

Chromosomal Location of Human Ortholog: 8q24

Cellular Component: axon terminus; cell soma; cytoplasm; cytosol; dendritic spine; endoplasmic reticulum; membrane; synapse; synaptic vesicle; terminal bouton

Molecular Function: heme binding; iron ion binding; oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen

Biological Process: adrenal gland development; androgen catabolic process; androgen metabolic process; bile acid metabolic process; biphenyl metabolic process; C21-steroid hormone metabolic process; dibenzo-p-dioxin metabolic process; estrogen biosynthetic process; female genitalia development; female gonad development; granulocyte differentiation; hippocampus development; hypothalamus development; luteinization; male gonad development; mammary gland development; melatonin metabolic process; negative regulation of bone resorption; negative regulation of chronic inflammatory response; phthalate metabolic process; regulation of synapse structure or activity; response to cesium ion; response to drug; response to estradiol; response to estrogen; response to herbicide; response to ionizing radiation; response to metal ion; response to morphine; response to organic cyclic compound; response to testosterone stimulus; uterus development

Research Articles on ARO

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Product Notes

The ARO cyp19a1 (Catalog #AAA2090521) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Aromatase (ARO) reacts with Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Aromatase (ARO) can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP). Western blotting: 0.2-2ug/mL;1:250-2500 Immunohistochemistry: 5-20ug/mL;1:25-100 Immunocytochemistry: 5-20ug/mL;1:25-100 Optimal working dilutions must be determined by end user. . Researchers should empirically determine the suitability of the ARO cyp19a1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Aromatase (ARO), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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