Human IP-10 ELISA Kit | IP-10 elisa kit

IP-10 (Human) OmniKine ELISA Kit

Cat. Num. AAA9501871

• Gene Names: CXCL10; C7; IFI10; INP10; IP-10; crg-2; mob-1; SCYB10; gIP-10
• Reactivity: Human
• Synonyms: IP-10; IP-10 (Human) OmniKine ELISA Kit; C-X-C motif chemokine 10; 10 kDa interferon gamma-induced protein; Gamma-IP10; Small-inducible cytokine B10; INP10; SCYB10; IP-10 elisa kit

• Reactivity: Human
• Specificity: The OmniKine™ Human IP-10 ELISA is capable of recognizing both recombinant and naturally produced Human IP-10 proteins. The antigens listed below were tested at 50 ng/ml and did not exhibit significant cross reactivity or interference.
• Form/Format: 12 x 8-Well Microstrips
• Sequence Length: 98

• Assay Type: Quantitative Sandwich
• Preparation and Storage: Store at 4 degree C for 6 months.

Testing Data

(The OmniKine Human IP-10 ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Human IP-10 proteins within the range of 8-2000 pg/ml.)

Related Product Information for IP-10 elisa kit

Principle of the Assay: The OmniKine™ Human IP-10 ELISA Kit contains the components necessary for quantitative determination of natural orrecombinant Human IP-10 concentrations within any experimental sample including cell lysates, serum and plasma. This particular immunoassay utilizes the quantitative technique of a "Sandwich" Enzyme-Linked Immunosorbent Assay (ELISA) where the target protein (antigen) isbound in a "sandwich" format by the primary capture antibodies coated to each well-bottom and the secondary detection antibodies added subsequently by the investigator. The capture antibodies coated to the bottom of each well are specific for a particular epitope on Human IP-10 while the user-added detection antibodies bind to epitopes on the captured target protein. Amid each step of the procedure, a series of wash steps must be performed to ensure the elimination of non-specific binding between proteins to other proteins or to the solid phase. After incubation and "sandwiching" of the target antigen, a peroxidase enzyme is conjugated to the constant heavy chain of the secondary antibody (either covalently or via Avidin/Streptavidin-Biotin interactions), allowing for a colorimetric reaction to ensue upon substrate addition. When the substrate TMB (3, 3', 5, 5'-Tetramethylbenzidine) is added, the reaction catalyzed by peroxidase yields a blue color that is representative of the antigen concentration. Upon sufficient color development, the reaction can be terminated through addition of Stop Solution (2 N Sulfuric Acid) where the color of the solution will turn yellow. The absorbance of each well can then be read by a spectrophotometer, allowing for generation of a standard curve and subsequent determination of protein concentration.

Background/Introduction: Human IP-10, most commonly known as the C-X-C Motif Chemokine 10 or Interferon-gamma Induced Protein 10 is an 8.7 kDa protein consisting of 98 residues after initial expression from the gene CXCL10 at locus 4q21 on chromosome 4. The overall structural composition of IP-10 includes 4 beta strands ranging from 3 to 7 residues each connected by beta turns, followed by a 9 residue helix towards the C-terminus of the cytokine. After post translational processing, the 21 residue signal sequence is removed from the actual 77 residue IP-10 protein via proteolytic cleavage. Within the IP-10 protein, the CXCL-10 (1-73) peptide is produced by proteolytic cleavage after secretion from keratinocytes. IP-10 is known to be a chemokine of the C-X-C subfamily and ligand for the receptor CXCR3. High affinity binding with the cell surface receptor CXCR3 results in pleiotropic effects, including chemotactic stimulation of monocytes and macrophages, natural killer and T-cell migration, as well as modulation of adhesion molecule expression, inhibition of bone marrow colony formation and angiogenesis. IP-10 has been implicated as a key mediator in the Interferon-gamma (IFN-gamma) response as its expression is able to be rapidly increased by a variety of cell types when exposed to IFN-gamma.

NCBI and Uniprot Product Information

• NCBI GI #: 149999382
• NCBI GeneID: 3627
• NCBI Accession #: NP_001556.2
• NCBI GenBank Nucleotide #: NM_001565.3
• UniProt Accession #: P02778; Q96QJ5
• Molecular Weight: 10,881 Da
• NCBI Official Full Name: C-X-C motif chemokine 10
• NCBI Official Synonym Full Names: C-X-C motif chemokine ligand 10
• NCBI Official Symbol: CXCL10
• NCBI Official Synonym Symbols: C7; IFI10; INP10; IP-10; crg-2; mob-1; SCYB10; gIP-10
• NCBI Protein Information: C-X-C motif chemokine 10
• UniProt Protein Name: C-X-C motif chemokine 10
• UniProt Gene Name: CXCL10
• UniProt Synonym Gene Names: INP10; SCYB10; Gamma-IP10; IP-10

NCBI Description

This antimicrobial gene encodes a chemokine of the CXC subfamily and ligand for the receptor CXCR3. Binding of this protein to CXCR3 results in pleiotropic effects, including stimulation of monocytes, natural killer and T-cell migration, and modulation of adhesion molecule expression. [provided by RefSeq, Sep 2014]

Uniprot Description

CXCL10: Chemotactic for monocytes and T-lymphocytes. Binds to CXCR3. Belongs to the intercrine alpha (chemokine CxC) family.

Protein type: Chemokine; Motility/polarity/chemotaxis; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 4q21.1

Cellular Component: external side of plasma membrane; extracellular region; extracellular space

Molecular Function: cAMP-dependent protein kinase regulator activity; chemokine activity; CXCR3 chemokine receptor binding; heparin binding; protein binding; receptor binding

Biological Process: blood circulation; cell surface receptor signaling pathway; cell-cell signaling; chemotaxis; defense response to virus; endothelial cell activation; G-protein coupled receptor signaling pathway; immune response; inflammatory response; muscle organ development; negative regulation of angiogenesis; negative regulation of myoblast differentiation; positive regulation of cAMP metabolic process; positive regulation of cell proliferation; positive regulation of release of sequestered calcium ion into cytosol; positive regulation of transcription from RNA polymerase II promoter; regulation of cell proliferation; regulation of protein kinase activity; response to cold; response to gamma radiation; response to vitamin D; signal transduction