Human Interleukin enhancer-binding factor 3 (ILF3) ELISA Kit | ILF3 elisa kit

Human Interleukin enhancer-binding factor 3 (ILF3) ELISA Kit

Cat. Num. AAA925724

• Gene Names: ILF3; CBTF; DRBF; MMP4; MPP4; NF90; NFAR; NF110; NF90a; NF90b; NFAR2; TCP80; DRBP76; NF110b; NFAR-1; TCP110; MPHOSPH4; NF-AT-90
• Reactivity: Human
• Synonyms: Interleukin enhancer-binding factor 3 (ILF3); Human Interleukin enhancer-binding factor 3 (ILF3) ELISA Kit; interleukin enhancer binding factor 3; 90kDa; CBTF; DRBF; DRBP76; MMP4; MPHOSPH4; MPP4; NF-AT-90; NF110; NF90; NFAR; NFAR-1; NFAR2; TCP110; TCP80; M-phase phosphoprotein 4|double-stranded RNA-binding protein; 76 kD|dsRNA binding protein NFAR-2/; ILF3 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of human ILF3. No significant cross-reactivity or interference between human ILF3 and analogues was observed.
• Sequence Length: 706

• Samples: Serum, plasma, tissue homogenates, Cell lysates
• Assay Type: Sandwich
• Detection Range: 18.75 pg/ml-1200 pg/ml.
• Sensitivity: The minimum detectable dose of human ILF3 is typically less than 4.69 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
• Precision: Intra-assay Precision (Precision within an assay) : CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. ; Inter-assay Precision (Precision between assays) : CV%<10% Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Related Product Information for ILF3 elisa kit

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for ILF3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ILF3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ILF3 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ILF3 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 212549555
• NCBI GeneID: 3609
• NCBI Accession #: NP_001131145.1
• NCBI GenBank Nucleotide #: NM_001137673.1
• UniProt Accession #: Q12906; O43409; Q6P1X1; Q86XY7; Q99544; Q99545; Q9BZH4; Q9BZH5; Q9NQ95; Q9NQ96; Q9NQ97; A8K6F2
• Molecular Weight: 95,338 Da
• NCBI Official Full Name: interleukin enhancer-binding factor 3 isoform e
• NCBI Official Synonym Full Names: interleukin enhancer binding factor 3, 90kDa
• NCBI Official Symbol: ILF3
• NCBI Official Synonym Symbols: CBTF; DRBF; MMP4; MPP4; NF90; NFAR; NF110; NF90a; NF90b; NFAR2; TCP80; DRBP76; NF110b; NFAR-1; TCP110; MPHOSPH4; NF-AT-90
• NCBI Protein Information: interleukin enhancer-binding factor 3; M-phase phosphoprotein 4; translational control protein 80; dsRNA binding protein NFAR-2/MPP4; nuclear factor associated with dsRNA; double-stranded RNA-binding protein, 76 kD; nuclear factor of activated T-cells 90 kDa; nuclear factor of activated T-cells, 90 kD
• UniProt Protein Name: Interleukin enhancer-binding factor 3
• Protein Family: Interleukin enhancer-binding factor
• UniProt Gene Name: ILF3
• UniProt Synonym Gene Names: DRBF; MPHOSPH4; NF90; DRBP76; MPP4; NFAR; NF-AT-90; TCP80
• UniProt Entry Name: ILF3_HUMAN

NCBI Description

This gene encodes a double-stranded RNA (dsRNA) binding protein that complexes with other proteins, dsRNAs, small noncoding RNAs, and mRNAs to regulate gene expression and stabilize mRNAs. This protein was first discovered to be a subunit of the nuclear factor of activated T-cells (NFAT); a transcription factor required for T-cell expression of interleukin 2. NFAT is a heterodimer of 45 kDa and 90 kDa proteins, the larger of which is the product of this gene. These proteins have been shown to affect the redistribution of nuclear mRNA to the cytoplasm. Knockdown of NF45 or NF90 protein retards cell growth; possibly by inhibition of mRNA stabilization. In contrast, an isoform (NF110) of this gene that is predominantly restricted to the nucleus has only minor effects on cell growth when its levels are reduced. Alternative splicing results in multiple transcript variants encoding distinct isoforms.[provided by RefSeq, Nov 2008]

Uniprot Description

NFAT90: a double-stranded RNA (dsRNA)-binding protein implicated in the regulation of gene expression . Phosphorylated in a double-stranded RNA-dependent manner possibly by PKR. Five different splice-variant isoforms have been described.

Protein type: DNA-binding; Transcription factor; RNA-binding; Nucleolus

Chromosomal Location of Human Ortholog: 19p13.2

Cellular Component: nucleoplasm; mitochondrion; membrane; cytoplasm; nucleolus; ribonucleoprotein complex; nucleus

Molecular Function: protein binding; DNA binding; RNA binding; double-stranded RNA binding

Biological Process: negative regulation of viral genome replication; transcription, DNA-dependent; negative regulation of translation; positive regulation of transcription, DNA-dependent; negative regulation of transcription, DNA-dependent; protein amino acid phosphorylation; defense response to virus