Rabbit Rab4 Polyclonal Antibody | anti-RAB5A antibody

Rab4 Antibody: Alkaline Phosphatase

Cat. Num. AAA806049

• Gene Names: RAB5A; RAB5
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunofluorescence, Immunocytochemistry
• Synonyms: Rab4; Polyclonal Antibody; Rab4 Antibody: Alkaline Phosphatase; Oncogene Rab4; Rab4A; Ras related protein; anti-RAB5A antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Form/Format: Rabbit Antiserum
• Sequence Length: 201

• Applicable Applications for anti-RAB5A antibody: Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)
• Application Notes: 1:1000-2000 (WB), 1:150 (ICC)
• Conjugate: Alkaline Phosphatase
• Storage Buffer: Rabbit Antiserum
• Preparation and Storage: -20 degree C

Western Blot (WB)

(Western blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of Rab4 protein using Rabbit Anti-Rab4 Polyclonal Antibody (SPC-141). Load: 15 ugprotein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Rabbit Anti-Rab4 Polyclonal Antibody (SPC-141) at 1:1000 for 2 hours at RT. Secondary Antibody: Donkey Anti-Rabbit IgG: HRP for 1 hour at RT.)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis using Rabbit Anti-Rab4 Polyclonal Antibody (SPC-141). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative Solution. Primary Antibody: Rabbit Anti-Rab4 Polyclonal Antibody (SPC-141) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:50 for 1 hour at RT. Localization: Epidermis (cell-cell border and cytoplasmic), hair follicles and muscle.)

Immunocytochemistry/Immunofluorescence (ICC/IF)

(Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Rab4 Polyclonal Antibody (SPC-141). Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol at -20C for 10 minutes. Primary Antibody: Rabbit Anti-Rab4 Polyclonal Antibody (SPC-141) at 1:100 for 12 hours at 4 degree C. Secondary Antibody: FITC Goat Anti-Rabbit at 1:50 for 1-2 hours at RT in dark. Localization: String nuclear and cytoplasmic staining.)

Related Product Information for anti-RAB5A antibody

Background Info: Detects ~26kDa.

Scientific Background: Rab4 is a 25kDa member of the Rab family of small guanosine triphosphatases (GTPases), Ras superfamily. Rab GTPases are central regulators of membrane trafficking in the eukaryotic cell. Their regulatory capacity depends on their ability to cycle between the GDP -bound inactive and GTP-bound active states. This conversion is regulated by GDP/GTP exchange factors (GEPs), GDP dissociation inhibitors (GDIs) and GTPase-activating proteins (GAPs) (1, 2). Activation of a Rab protein is coupled to its association with intracellular membranes, allowing it to recruit downstream effector proteins to the cytoplasmic surface of a sub-cellular compartment (3). Through these proteins, Rab GTPases regulate vesicle formation, actin- and tubulin-dependent vesicle movement, and membrane fusion(1). Rab proteins contain conserved regions involved in guanine-nucleotide binding, and hyper-variable COHO-terminal domains with a cysteine motif implicated in sub-cellular targeting. Post-translational modification of the cysteine motif with one or two geranylgeranyl groups is essential for the membrane association and correct intracellular localization of Rab proteins (3). Each Rab shows a characteristic sub-cellular distribution (4). In particular, over-expression of Rab4 causes a redistribution of receptors on plasma membrane versus endocytic compartments. The presence of excessive Rab4 leads to the accumulation of tranferrin receptors in non-acidic, post-endosomal recycling vesicles considered an intermediate compartment between endosomes and plasma membranes. Rab4 also plays a role in the translocation of glucose transporter (Glu4) in adipocytes in response to insulin (5). Mediating the association of Rab4 with transferring receptor-containing early endosomes takes place through the geranylgeranyl groups at its carboxyl-terminus. Membrane association is also cell cycle dependent, as phosphorylation at its c-terminal cdc2 kinase consensus sequence in mitotic cells leads to dissociation of Rab4 into the cytosol (6).

Product Categories/Family for anti-RAB5A antibody

Cell Signaling; Neuroscience; Organelle Markers

References

1. Stenmark H., and Olkkonen V.M. (2001) Genome Biol. 2: 3007.1-3007.7. 2. Takai Y., et al. (2001) Physiol. Rev. 8:, 153-208. 3. Ali B.R., et al. (2004) J. Cell Sci. 117: 6401-6412. 4. Zerial M., and McBride H. (2001) Nat. Rev. Mol. Cell Biol. 2: 107-117. 5. Cormont M., et al. (1996) Mol Cell Biology. 16: 6879-6886. 6. Ayad N., Hull M., and Mellman I. (1997) EMBO 16: 4497-4507. 7. Van der Sluijs, P. et l. (1992) The EMBO Journal 11(12), 4379-4389.
1. Ballmer-Hofer, K., Andersson, A.E., Ratcliffe, L.E. and Berger, P. (2011). Neuropilin-1 promotes VEGFR-2 trafficking through Rab11 vesicles thereby specifying signal output. Blood. 118 (3), 816-826. doi: 10.1182/blood-2011-01-328773.

NCBI and Uniprot Product Information

• NCBI GeneID: 5868
• UniProt Accession #: P20339; Q6FI44; B4DJA5
• Molecular Weight: 22,178 Da
• NCBI Official Synonym Full Names: RAB5A, member RAS oncogene family
• NCBI Official Symbol: RAB5A
• NCBI Official Synonym Symbols: RAB5
• NCBI Protein Information: ras-related protein Rab-5A
• UniProt Protein Name: Ras-related protein Rab-5A
• Protein Family: Ras-related protein
• UniProt Gene Name: RAB5A
• UniProt Synonym Gene Names: RAB5
• UniProt Entry Name: RAB5A_HUMAN

Uniprot Description

RAB5A: Required for the fusion of plasma membranes and early endosomes. Contributes to the regulation of filopodia extension. Binds EEA1. Interacts with RIN1 and GAPVD1, which regulate its pathway, probably by acting as a GEF. Interacts with ALS2CL, RABEP1, SUN2, ZFYVE20 and RUFY1. Interacts with SGSM1 and SGSM3. Interacts with PIK3CB. Regulated by guanine nucleotide exchange factors (GEFs) which promote the exchange of bound GDP for free GTP. Belongs to the small GTPase superfamily. Rab family.

Protein type: G protein; G protein, monomeric, Rab; Motility/polarity/chemotaxis; G protein, monomeric

Chromosomal Location of Human Ortholog: 3p24-p22

Cellular Component: zymogen granule membrane; protein complex; early endosome; dendrite; terminal button; phagocytic vesicle; cytosol; lipid raft; actin cytoskeleton; ruffle; synaptic vesicle; recycling endosome; cell soma; perinuclear region of cytoplasm; endocytic vesicle; axon; cytoplasm; plasma membrane; melanosome; endosome membrane; nerve terminal; endosome

Molecular Function: GTPase activity; protein binding; GDP binding; GTP binding; GDP-dissociation inhibitor binding

Biological Process: regulation of long-term neuronal synaptic plasticity; intracellular protein transport; regulation of endosome size; early endosome to late endosome transport; positive regulation of smooth muscle cell proliferation; regulation of endocytosis; regulation of filopodium formation; endocytosis; blood coagulation; positive regulation of smooth muscle cell migration; Rab protein signal transduction; positive regulation of exocytosis