Rabbit anti-Human Interleukin enhancer-binding factor 3 Polyclonal Antibody | anti-ILF3 antibody

Rabbit anti-human Interleukin enhancer-binding factor 3 polyclonal Antibody(ILF3)

Cat. Num. AAA1497470

• Gene Names: ILF3; CBTF; DRBF; MMP4; MPP4; NF90; NFAR; NF110; NF90a; NF90b; NFAR2; TCP80; DRBP76; NF110b; NFAR-1; TCP110; MPHOSPH4; NF-AT-90
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Antigen Affinity Purified
• Synonyms: Interleukin enhancer-binding factor 3; Polyclonal Antibody; Rabbit anti-human Interleukin enhancer-binding factor 3 polyclonal Antibody(ILF3); Double-stranded RNA-binding protein 76; DRBP76; M-phase phosphoprotein 4; MPP4; Nuclear factor associated with dsRNA; NFAR; Nuclear factor of activated T-cells 90 kDa; NF-AT-90; Translational control protein 80; TCP80; anti-ILF3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Antigen Affinity Purified
• Sequence Length: 706

• Applicable Applications for anti-ILF3 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Recommended dilution: WB:1:200-1000; IHC:1:20-1:200
• Storage Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Conjugate: Non-conjugated
• Immunogen: Recombinant human Interleukin enhancer-binding factor 3 protein (785-894aa)
• Santa Cruz Alternative: Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-102035
• Preparation and Storage: Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.

Western Blot (WB)

(All lanes: Interleukin enhancer-binding factor 3 antibody at 3ug/ml+HepG2 whole cell lysateSecondaryGoat polyclonal to rabbit at 1/10000 dilutionPredicted band size: 96,77,83,76,75 kDaObserved band size: 95 kDa)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human cervical cancer using MBS1497470 at dilution 1:100)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human kidney using MBS1497470 at dilution 1:100)

Related Product Information for anti-ILF3 antibody

May facilitate double-stranded RNA-regulated gene expression at the level of post-transcription. Can act as a translation inhibitory protein which binds to coding sequences of acid beta-glucosidase (GCase) and other mRNAs and functions at the initiation phase of GCase mRNA translation, probably by inhibiting its binding to polysomes. Can regulate protein arginine N-methyltransferase 1 activity. May regulate transcription of the IL2 gene during T-cell activation. Can promote the formation of stable DNA-dependent protein kinase holoenzyme complexes on DNA. The phosphorylated form at Thr-188 and Thr-315, in concert with EIF2AK2/PKR can inhibit vesicular stomatitis virus (VSV) replication (By similarity).

NCBI and Uniprot Product Information

• NCBI GI #: 212549555
• NCBI GeneID: 3609
• NCBI Accession #: NP_001131145.1
• NCBI GenBank Nucleotide #: NM_001137673.1
• UniProt Accession #: Q12906; O43409; Q6P1X1; Q86XY7; Q99544; Q99545; Q9BZH4; Q9BZH5; Q9NQ95; Q9NQ96; A8K6F2; G5E9M5
• Molecular Weight: 95,808 Da
• NCBI Official Full Name: interleukin enhancer-binding factor 3 isoform e
• NCBI Official Synonym Full Names: interleukin enhancer binding factor 3
• NCBI Official Symbol: ILF3
• NCBI Official Synonym Symbols: CBTF; DRBF; MMP4; MPP4; NF90; NFAR; NF110; NF90a; NF90b; NFAR2; TCP80; DRBP76; NF110b; NFAR-1; TCP110; MPHOSPH4; NF-AT-90
• NCBI Protein Information: interleukin enhancer-binding factor 3
• UniProt Protein Name: Interleukin enhancer-binding factor 3
• Protein Family: Interleukin enhancer-binding factor
• UniProt Gene Name: ILF3
• UniProt Synonym Gene Names: DRBF; MPHOSPH4; NF90; DRBP76; MPP4; NFAR; NF-AT-90; TCP80
• UniProt Entry Name: ILF3_HUMAN

NCBI Description

This gene encodes a double-stranded RNA (dsRNA) binding protein that complexes with other proteins, dsRNAs, small noncoding RNAs, and mRNAs to regulate gene expression and stabilize mRNAs. This protein (NF90, ILF3) forms a heterodimer with a 45 kDa transcription factor (NF45, ILF2) required for T-cell expression of interleukin 2. This complex has been shown to affect the redistribution of nuclear mRNA to the cytoplasm. Knockdown of NF45 or NF90 protein retards cell growth, possibly by inhibition of mRNA stabilization. In contrast, an isoform (NF110) of this gene that is predominantly restricted to the nucleus has only minor effects on cell growth when its levels are reduced. Alternative splicing results in multiple transcript variants encoding distinct isoforms.[provided by RefSeq, Dec 2014]

Uniprot Description

NFAT90: a double-stranded RNA (dsRNA)-binding protein implicated in the regulation of gene expression . Phosphorylated in a double-stranded RNA-dependent manner possibly by PKR. Five different splice-variant isoforms have been described.

Protein type: RNA-binding; Transcription factor; Nucleolus; DNA-binding

Chromosomal Location of Human Ortholog: 19p13.2

Cellular Component: cytoplasm; membrane; mitochondrion; nucleolus; nucleoplasm; nucleus; ribonucleoprotein complex

Molecular Function: DNA binding; double-stranded RNA binding; protein binding; RNA binding

Biological Process: defense response to virus; negative regulation of transcription, DNA-dependent; negative regulation of translation; negative regulation of viral genome replication; positive regulation of transcription, DNA-dependent; protein amino acid phosphorylation; transcription, DNA-dependent