Rabbit ILF3 Polyclonal Antibody | anti-ILF3 antibody

ILF3 Antibody

Cat. Num. AAA9213988

• Gene Names: ILF3; CBTF; DRBF; MMP4; MPP4; NF90; NFAR; NF110; NF90a; NF90b; NFAR2; TCP80; DRBP76; NF110b; NFAR-1; TCP110; MPHOSPH4; NF-AT-90
• Reactivity: Human, mouse, rat
• Applications: Western Blot, ELISA
• Purity: Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: ILF3; Polyclonal Antibody; ILF3 Antibody; Interleukin enhancer-binding factor 3; Double-stranded RNA-binding protein 76; DRBP76; M-phase phosphoprotein 4; MPP4; Nuclear factor associated with dsRNA; NFAR; Nuclear factor of activated T-cells 90 kDa; NF-AT-90; Translational control protein 80; TCP80; DRBF; MPHOSPH4; NF90; anti-ILF3 antibody

• Host: Rabbit
• Reactivity: Human, mouse, rat
• Clonality: Polyclonal
• Purity/Purification: Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: 0.01M PBS, pH 7.2, 0.1% Sodium azide, Glycerol 50%
• Concentration: 1mg/ml (varies by lot)
• Sequence Positions: 301-360
• Sequence Length: 706

• Applicable Applications for anti-ILF3 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB~~ 1:1000
• Antigen Type: synthetic peptide
• Antigen Source: KLH conjugated synthetic peptide derived from human ILF3
• Preparation and Storage: Store at -20 degree C.Stable for 12 months from date of receipt

Western Blot (WB)

(All lanes : Anti-ILF3 Antibody at 1:1000 dilutionLane 1: Hela whole cell lysatesLane 2: K562 whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilutionPredicted band size : 95 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Related Product Information for anti-ILF3 antibody

May facilitate double-stranded RNA-regulated gene expression at the level of post-transcription. Can act as a translation inhibitory protein which binds to coding sequences of acid beta-glucosidase (GCase) and other mRNAs and functions at the initiation phase of GCase mRNA translation, probably by inhibiting its binding to polysomes. Can regulate protein arginine N- methyltransferase 1 activity. May regulate transcription of the IL2 gene during T-cell activation. Can promote the formation of stable DNA-dependent protein kinase holoenzyme complexes on DNA. The phosphorylated form at Thr-188 and Thr-315, in concert with EIF2AK2/PKR can inhibit vesicular stomatitis virus (VSV) replication (By similarity).

Product Categories/Family for anti-ILF3 antibody

Signal Transduction

References

Kao P.N.,et al.J. Biol. Chem. 269:20691-20699(1994).
Patel R.C.,et al.J. Biol. Chem. 274:20432-20437(1999).
Xu Y.-H.,et al.Mol. Genet. Metab. 68:441-454(1999).
Duchange N.,et al.Gene 261:345-353(2000).
Saunders L.R.,et al.J. Biol. Chem. 276:32300-32312(2001).

NCBI and Uniprot Product Information

• NCBI GI #: 212549555
• NCBI GeneID: 3609
• NCBI Accession #: NP_001131145.1
• NCBI GenBank Nucleotide #: NM_001137673.1
• UniProt Accession #: Q12906; O43409; Q6P1X1; Q86XY7; Q99544; Q99545; Q9BZH4; Q9BZH5; Q9NQ95; Q9NQ96; A8K6F2; G5E9M5
• Molecular Weight: 95338
• NCBI Official Full Name: interleukin enhancer-binding factor 3 isoform e
• NCBI Official Synonym Full Names: interleukin enhancer binding factor 3, 90kDa
• NCBI Official Symbol: ILF3
• NCBI Official Synonym Symbols: CBTF; DRBF; MMP4; MPP4; NF90; NFAR; NF110; NF90a; NF90b; NFAR2; TCP80; DRBP76; NF110b; NFAR-1; TCP110; MPHOSPH4; NF-AT-90
• NCBI Protein Information: interleukin enhancer-binding factor 3
• UniProt Protein Name: Interleukin enhancer-binding factor 3
• Protein Family: Interleukin enhancer-binding factor
• UniProt Gene Name: ILF3
• UniProt Synonym Gene Names: DRBF; MPHOSPH4; NF90; DRBP76; MPP4; NFAR; NF-AT-90; TCP80
• UniProt Entry Name: ILF3_HUMAN

NCBI Description

This gene encodes a double-stranded RNA (dsRNA) binding protein that complexes with other proteins, dsRNAs, small noncoding RNAs, and mRNAs to regulate gene expression and stabilize mRNAs. This protein (NF90, ILF3) forms a heterodimer with a 45 kDa transcription factor (NF45, ILF2) required for T-cell expression of interleukin 2. This complex has been shown to affect the redistribution of nuclear mRNA to the cytoplasm. Knockdown of NF45 or NF90 protein retards cell growth, possibly by inhibition of mRNA stabilization. In contrast, an isoform (NF110) of this gene that is predominantly restricted to the nucleus has only minor effects on cell growth when its levels are reduced. Alternative splicing results in multiple transcript variants encoding distinct isoforms.[provided by RefSeq, Dec 2014]

Uniprot Description

NFAT90: a double-stranded RNA (dsRNA)-binding protein implicated in the regulation of gene expression . Phosphorylated in a double-stranded RNA-dependent manner possibly by PKR. Five different splice-variant isoforms have been described.

Protein type: Transcription factor; Nucleolus; DNA-binding; RNA-binding

Chromosomal Location of Human Ortholog: 19p13.2

Cellular Component: nucleoplasm; mitochondrion; membrane; cytoplasm; nucleolus; nucleus; ribonucleoprotein complex

Molecular Function: protein binding; DNA binding; RNA binding; double-stranded RNA binding

Biological Process: negative regulation of viral genome replication; transcription, DNA-dependent; positive regulation of transcription, DNA-dependent; negative regulation of translation; negative regulation of transcription, DNA-dependent; protein amino acid phosphorylation; defense response to virus