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Typical Testing Data/Standard Curve (for reference only) (Fig.1. Human Interleukin-36 gamma (IL1F9) Standard Curve.)

Human Interleukin-36 gamma (IL1F9) ELISA Kit | IL1F9 elisa kit

Human Interleukin-36 gamma (IL1F9) ELISA Kit

Gene Names
IL36G; IL1E; IL1F9; IL1H1; IL-1F9; IL-1H1; IL1RP2; IL-1RP2
Reactivity
Human
Synonyms
Interleukin-36 gamma (IL1F9); Human Interleukin-36 gamma (IL1F9) ELISA Kit; IL1F9; IL-1F9; IL-1H1; IL-1RP2; IL1E; IL1H1; IL1RP2; IL-1 related protein 2; IL-1(EPSILON); IL-1-epsilon; interleukin 1-related protein 2; interleukin-1 epsilon; interleukin-1 homolog 1; IL1F9 elisa kit
Ordering
For Research Use Only!
Reactivity
Human
Specificity
Human Interleukin-36 gamma (IL1F9) ELISA Kit has high sensitivity and excellent specificity for detection of Human IL1F9. No significant cross-reactivity or interference between Human IL1F9 and analogues was observed.
Sequence Length
169
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

(Fig.1. Human Interleukin-36 gamma (IL1F9) Standard Curve.)

Typical Testing Data/Standard Curve (for reference only) (Fig.1. Human Interleukin-36 gamma (IL1F9) Standard Curve.)
Related Product Information for IL1F9 elisa kit
Description: This Human Interleukin-36 gamma (IL1F9) ELISA Kit employs a two-site sandwich ELISA to quantitate IL1F9 in samples. An antibody specific for IL1F9 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL1F9 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IL1F9 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IL1F9 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Background: IL1F9 encoded by this gene is a member of the interleukin 1 cytokine family. The activity of this cytokine is mediated by interleukin 1 receptor-like 2, and is specifically inhibited by interleukin 1 family, member 5. Interferon-gamma, tumor necrosis factor-alpha and interleukin 1, beta (IL1B) are reported to stimulate the expression of this cytokine in keratinocytes. The expression of this cytokine in keratinocytes can also be induced by a contact hypersensitivity reaction or herpes simplex virus infection. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2.Highly expressed in tissues containing epithelial cells: skin, lung, stomach and esophagus. In skin is expressed only in keratinocytes but not in fibroblasts, endothelial cells or melanocytes. Up-regulated in lesional psoriasis skin.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
interleukin-36 gamma isoform 1
NCBI Official Synonym Full Names
interleukin 36 gamma
NCBI Official Symbol
IL36G
NCBI Official Synonym Symbols
IL1E; IL1F9; IL1H1; IL-1F9; IL-1H1; IL1RP2; IL-1RP2
NCBI Protein Information
interleukin-36 gamma
UniProt Protein Name
Interleukin-36 gamma
UniProt Gene Name
IL36G
UniProt Synonym Gene Names
IL1E; IL1F9; IL1H1; IL1RP2; IL-1RP2; IL-1 epsilon; IL-1F9; IL-1H1
UniProt Entry Name
IL36G_HUMAN

NCBI Description

The protein encoded by this gene is a member of the interleukin 1 cytokine family. The activity of this cytokine is mediated by interleukin 1 receptor-like 2 (IL1RL2/IL1R-rp2), and is specifically inhibited by interleukin 1 family, member 5 (IL1F5/IL-1 delta). Interferon-gamma, tumor necrosis factor-alpha and interleukin 1, beta (IL1B) are reported to stimulate the expression of this cytokine in keratinocytes. The expression of this cytokine in keratinocytes can also be induced by a contact hypersensitivity reaction or herpes simplex virus infection. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. [provided by RefSeq, May 2019]

Uniprot Description

Il1f9: Function as an agonist of NF-kappa B activation through the orphan IL-1-receptor-related protein 2. Could constitute part of an independent signaling system analogous to interleukin-1 alpha (IL-1A), beta (IL-1B) receptor agonist and interleukin-1 receptor type I (IL-1R1), that is present in epithelial barriers and takes part in local inflammatory response. Belongs to the IL-1 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cytokine

Chromosomal Location of Human Ortholog: 2q12-q21

Cellular Component: extracellular space

Molecular Function: interleukin-1 receptor binding; cytokine activity

Biological Process: cell-cell signaling; cytokine and chemokine mediated signaling pathway; positive regulation of interleukin-6 production; innate immune response; negative regulation of myoblast differentiation; inflammatory response

Research Articles on IL1F9

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Product Notes

The Human IL1F9 il36g (Catalog #AAA9715906) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9715906 ELISA Kit recognizes Human IL1F9. It is sometimes possible for the material contained within the vial of "Interleukin-36 gamma (IL1F9), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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