Human Integrin Alpha 6 (ITGa6) ELISA Kit | ITGa6 elisa kit

Human Integrin Alpha 6 (ITGa6) ELISA Kit

Cat. Num. AAA2703123

• Gene Names: ITGA6; CD49f; VLA-6; ITGA6B
• Reactivity: Human
• Synonyms: Integrin Alpha 6 (ITGa6); Human Integrin Alpha 6 (ITGa6) ELISA Kit; CD49f; CD49-F; ITG-A6; ITGA6B; VLA-6; CD49 antigen-like family member F; Processed integrin alpha-6; ITGa6 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of ITGa6. No significant cross-reactivity or interference between ITGa6 and analogues was observed.
• Sequence Length: 1,097

• Samples: Human tissue homogenates, cell lysates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.054ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level ITGa6 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level ITGa6 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ITGa6 elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of ITGa6 in human tissue homogenates, cell lysates and other biological fluids.

Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to ITGa6. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to ITGa6. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain ITGa6, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of ITGa6 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 5726563
• NCBI GeneID: 3655
• UniProt Accession #: P23229; Q08443; Q0MRC7; Q14646; Q16508; Q53RX7; Q59HB7; B2RMU9; B4DG69; B4DKB8; C4AM96; G5E9H1
• Molecular Weight: 122,358 Da
• NCBI Official Full Name: integrin alpha 6
• NCBI Official Synonym Full Names: integrin subunit alpha 6
• NCBI Official Symbol: ITGA6
• NCBI Official Synonym Symbols: CD49f; VLA-6; ITGA6B
• NCBI Protein Information: integrin alpha-6
• UniProt Protein Name: Integrin alpha-6
• Protein Family: Integrin
• UniProt Gene Name: ITGA6
• UniProt Synonym Gene Names: Alpha6p

NCBI Description

The gene encodes a member of the integrin alpha chain family of proteins. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain that function in cell surface adhesion and signaling. The encoded preproprotein is proteolytically processed to generate light and heavy chains that comprise the alpha 6 subunit. This subunit may associate with a beta 1 or beta 4 subunit to form an integrin that interacts with extracellular matrix proteins including members of the laminin family. The alpha 6 beta 4 integrin may promote tumorigenesis, while the alpha 6 beta 1 integrin may negatively regulate erbB2/HER2 signaling. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2015]

Uniprot Description

Integrin alpha-6/beta-1 is a receptor for laminin on platelets. Integrin alpha-6/beta-4 is a receptor for laminin in epithelial cells and it plays a critical structural role in the hemidesmosome (). ITGA6:ITGB4 binds to NRG1 (via EGF domain) and this binding is essential for NRG1-ERBB signaling (PubMed:20682778). ITGA6:ITGB4 binds to IGF1 and this binding is essential for IGF1 signaling (PubMed:22351760).