Typical Testing Data/Standard Curve
Human Integral membrane protein 2C (ITM2C) ELISA Kit | ITM2C elisa kit
Human Integral membrane protein 2C (ITM2C) ELISA Kit
Gene Names
ITM2C; E25; BRI3; E25C; ITM3; BRICD2C
Reactivity
Human
Synonyms
Integral membrane protein 2C (ITM2C); Human Integral membrane protein 2C (ITM2C) ELISA Kit; ITM2C elisa kit
Reactivity
Human
Samples
Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type
Competitive
Detection Range
0.5-10ng/mL
Sensitivity
0.1ng/mL
Preparation and Storage
Store all reagents at 2-8 degree C.
Typical Testing Data/Standard Curve
Typical Testing Data/Standard Curve
Related Product Information for ITM2C elisa kit
Intended Uses: This ITM2C ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human ITM2C. This ELISA kit for research use only!
Principle of the Assay: ITM2C ELISA kit applies the competitive enzyme immunoassay technique utilizing a Polyclonal anti-ITM2C antibody and an ITM2C-HRP conjugate. The assay sample and buffer are incubated together with ITM2C-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ITM2C concentration since ITM2C from samples and ITM2C-HRP conjugate compete for the anti-ITM2C antibody binding site. Since the number of sites is limited, as more sites are occupied by ITM2C from the sample, fewer sites are left to bind ITM2C-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ITM2C concentration in each sample is interpolated from this standard curve.
Principle of the Assay: ITM2C ELISA kit applies the competitive enzyme immunoassay technique utilizing a Polyclonal anti-ITM2C antibody and an ITM2C-HRP conjugate. The assay sample and buffer are incubated together with ITM2C-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ITM2C concentration since ITM2C from samples and ITM2C-HRP conjugate compete for the anti-ITM2C antibody binding site. Since the number of sites is limited, as more sites are occupied by ITM2C from the sample, fewer sites are left to bind ITM2C-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ITM2C concentration in each sample is interpolated from this standard curve.
Product Categories/Family for ITM2C elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
25,921 Da
NCBI Official Full Name
Integral membrane protein 2C
NCBI Official Synonym Full Names
integral membrane protein 2C
NCBI Official Symbol
ITM2C
NCBI Official Synonym Symbols
E25; BRI3; E25C; ITM3; BRICD2C
NCBI Protein Information
integral membrane protein 2C; cerebral protein 14; transmembrane protein BRI3; integral membrane protein 3; BRICHOS domain containing 2C
UniProt Protein Name
Integral membrane protein 2C
Protein Family
UniProt Gene Name
ITM2C
UniProt Synonym Gene Names
BRI3; hucep-14; NPD018; PSEC0047
UniProt Entry Name
ITM2C_HUMAN
Uniprot Description
ITM2C: Negative regulator of beta amyloid peptide production. May inhibit the processing of APP by blocking its access to alpha- and beta-secretase. Binding to the beta-secretase-cleaved APP C- terminal fragment is negligible, suggesting that ITM2C is a poor gamma-secretase cleavage inhibitor. May play a role in TNF-induced cell death and neuronal differentiation. Belongs to the ITM2 family. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Membrane protein, integral
Chromosomal Location of Human Ortholog: 2q37
Cellular Component: Golgi apparatus; lysosomal membrane; perinuclear region of cytoplasm; lysosome; plasma membrane; integral to membrane
Molecular Function: protein binding; beta-amyloid binding; ATP binding
Biological Process: neuron differentiation
Research Articles on ITM2C
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Product Notes
The Human ITM2C itm2c (Catalog #AAA7243136) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7243136 ELISA Kit recognizes Human ITM2C. It is sometimes possible for the material contained within the vial of "Integral membrane protein 2C (ITM2C), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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