Human MUC2 ELISA Kit | MUC2 elisa kit

Human MUC2 (Mucin 2) ELISA Kit

Cat. Num. AAA2509166

• Gene Names: MUC2; MLP; SMUC; MUC-2
• Reactivity: Human
• Synonyms: MUC2; Human MUC2 (Mucin 2) ELISA Kit; MUC2 elisa kit

• Reactivity: Human
• Specificity: This kit recognizes Human MUC2 in samples. No significant cross-reactivity or interference between Human MUC2 and analogues was observed.
• Sequence Length: 5179

• Samples: Serum, plasma and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 0.31-20ng/mL
• Sensitivity: 0.19ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human MUC2 were tested 20 times on one plate, respectively.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human MUC2 were tested on 3 different plates, 20 replicates in each plate.
• Preparation and Storage: Store at 4 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for MUC2 elisa kit

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Human MUC2 concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human MUC2. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human MUC2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human MUC2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human MUC2. You can calculate the concentration of Human MUC2 in the samples by comparing the OD of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 557129045
• NCBI GeneID: 4583
• NCBI Accession #: NP_002448.3
• NCBI GenBank Nucleotide #: NM_002457.3
• UniProt Accession #: Q02817; Q14878
• Molecular Weight: 540,300 Da
• NCBI Official Full Name: mucin-2
• NCBI Official Synonym Full Names: mucin 2, oligomeric mucus/gel-forming
• NCBI Official Symbol: MUC2
• NCBI Official Synonym Symbols: MLP; SMUC; MUC-2
• NCBI Protein Information: mucin-2; intestinal mucin-2; mucin 2, intestinal/tracheal; mucin-like protein
• UniProt Protein Name: Mucin-2
• Protein Family: Mucin
• UniProt Gene Name: MUC2
• UniProt Synonym Gene Names: SMUC; MUC-2
• UniProt Entry Name: MUC2_HUMAN

NCBI Description

This gene encodes a member of the mucin protein family. Mucins are high molecular weight glycoproteins produced by many epithelial tissues. The protein encoded by this gene is secreted and forms an insoluble mucous barrier that protects the gut lumen. The protein polymerizes into a gel of which 80% is composed of oligosaccharide side chains by weight. The protein features a central domain containing tandem repeats rich in threonine and proline that varies between 50 and 115 copies in different individuals. Alternatively spliced transcript variants of this gene have been described, but their full-length nature is not known. [provided by RefSeq, Jul 2008]

Uniprot Description

MUC2: Coats the epithelia of the intestines, airways, and other mucus membrane-containing organs. Thought to provide a protective, lubricating barrier against particles and infectious agents at mucosal surfaces. Major constituent of both the inner and outer mucus layers of the colon and may play a role in excluding bacteria from the inner mucus layer.

Protein type: Secreted, signal peptide; Secreted; Extracellular matrix

Chromosomal Location of Human Ortholog: 11p15.5

Cellular Component: Golgi lumen

Molecular Function: protein binding

Biological Process: protein amino acid O-linked glycosylation; cellular protein metabolic process; O-glycan processing; maintenance of gastrointestinal epithelium; post-translational protein modification