Human Inhibin Beta C (INHbC) ELISA Kit | INHbC elisa kit

Human Inhibin Beta C (INHbC) ELISA Kit

Cat. Num. AAA455359

• Gene Names: INHBC; IHBC
• Reactivity: Human
• Synonyms: Inhibin Beta C (INHbC); Human Inhibin Beta C (INHbC) ELISA Kit; IHBC; INH-Bc; INHbC elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of INHbC. No significant cross-reactivity or interference between INHbC and analogues was observed.
• Sequence Length: 352

• Samples: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
• Assay Type: Sandwich
• Detection Range: 0.156-10ng/mL
• Sensitivity: 0.062ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level INHbC were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level INHbC were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for INHbC elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of INHbC in human serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to INHbC. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to INHbC. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain INHbC, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of INHbC in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 5031795
• NCBI GeneID: 3626
• NCBI Accession #: NP_005529.1
• NCBI GenBank Nucleotide #: NM_005538.3
• UniProt Accession #: P55103; A1L3Y2
• Molecular Weight: 38,238 Da
• NCBI Official Full Name: inhibin beta C chain preproprotein
• NCBI Official Synonym Full Names: inhibin beta C subunit
• NCBI Official Symbol: INHBC
• NCBI Official Synonym Symbols: IHBC
• NCBI Protein Information: inhibin beta C chain
• UniProt Protein Name: Inhibin beta C chain
• Protein Family: Inhibin
• UniProt Gene Name: INHBC

NCBI Description

This gene encodes a member of the TGF-beta (transforming growth factor-beta) superfamily of proteins. The encoded preproprotein is proteolytically processed to generate a subunit of homodimeric and heterodimeric activin complexes. The heterodimeric complex may function in the inhibition of activin A signaling. Transgenic mice overexpressing this gene exhibit defects in testis, liver and prostate. [provided by RefSeq, Aug 2016]

Uniprot Description

Inhibins and activins inhibit and activate, respectively, the secretion of follitropin by the pituitary gland. Inhibins/activins are involved in regulating a number of diverse functions such as hypothalamic and pituitary hormone secretion, gonadal hormone secretion, germ cell development and maturation, erythroid differentiation, insulin secretion, nerve cell survival, embryonic axial development or bone growth, depending on their subunit composition. Inhibins appear to oppose the functions of activins.