Western Blot (WB)
(Anti-PGM2L1 rabbit polyclonal antibody at 1:500 dilution Lane A: HL60 Whole Cell Lysate Lane B: Caco-2 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:70 kDa)
Rabbit anti-Human PGM2L1 Polyclonal Antibody | anti-PGM2L1 antibody
Anti-PGM2L1 Antibody, Rabbit Polyclonal
Reactivity
Human
Applications
Western Blot
Purity
Protein A & Antigen Affinity
Synonyms
PGM2L1; Polyclonal Antibody; Anti-PGM2L1 Antibody; Rabbit Polyclonal; PGM2L1 Antibody; Rabbit PAb; Antigen Affinity Purified; Anti-BM32A Antibody; Anti-PMMLP Antibody; anti-PGM2L1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human PGM2L1
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; PBS, pH7.0 with 0.03% Proclin300
Applicable Applications for anti-PGM2L1 antibody
Western Blot (WB)
Application Notes
WB: 1:500-1:1000
Immunogen
E Coli-derived Human PGM2L1 fragment
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with E Coli-derived Human PGM2L1 fragment, and purified by antigen affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
(Anti-PGM2L1 rabbit polyclonal antibody at 1:500 dilution Lane A: HL60 Whole Cell Lysate Lane B: Caco-2 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:70 kDa)
Western Blot (WB)
(Anti-PGM2L1 rabbit polyclonal antibody at 1:500 dilution Lane A: HL60 Whole Cell Lysate Lane B: Caco-2 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:70 kDa)
Related Product Information for anti-PGM2L1 antibody
The human genome contains five proteins in the alpha-D-phosphohexomutase superfamily. They are epitomized by phosphoglucomutase 1 (PGM1), a critical enzyme in metabolism that regulates glucose homeostasis through the interconversion of glucose 1-phosphate and glucose 6-phosphate. Four other proteins, known as PGM2, PGM2L1, PGM3, and PGM5, are sequence related to PGM1, but differ in their substrate preferences or mechanism, and have distinct biological roles. PGM2 has phosphopentomutase activity, while PGM2L1 (for PGM2-like 1) is specialized for glucose 1, 6-bisphosphate (G16P) synthase activity. PGM3 is an N-acetylglucosamine phosphomutase and participates in hexosamine biosynthesis. PGM5 is reported to lack enzyme activity, but has established structural roles in myofibril assembly and maintenance, and is a binding partner for dystrophin. All of these proteins belong to the alpha-D-phosphohexomutase superfamily, which is ubiquitous in all kingdoms of life. Members of the superfamily typically catalyze an intramolecular phosphoryl transfer, often from the 1-to the 6-position of a hexose. Major sub-groups within the enzyme superfamily differ in their preferences for the sugar moiety of their substrates, which include glucose, mannose, glucosamine and N-acetyl glucosamine.
References
Veiga-da-Cunha M, et al. (2008) Mammalian phosphomannomutase pmm1 is the brain imp-sensitive glucose-1, 6-bisphosphatase. J Biol Chem 283 (49): 33988-33993.Muenks AG, et al. (2017) Sequence-structure relationships, expression profiles, and disease-associated mutations in the paralogs of phosphoglucomutase 1. PLoS One 12 (8): e0183563.Maliekal P, et al. (2007) Molecular identification of mammalian phosphopentomutase and glucose-1, 6-bisphosphate synthase, two members of the alpha-d-phosphohexomutase family. J Biol Chem 282 (44): 31844-31851.
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Product Notes
The PGM2L1 (Catalog #AAA8109693) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-PGM2L1 Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PGM2L1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:500-1:1000. Researchers should empirically determine the suitability of the PGM2L1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PGM2L1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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