Human IL-28B ELISA Kit | IL-28B elisa kit

IL-28B (Human) OmniKine ELISA Kit

Cat. Num. AAA9501973

• Gene Names: IFNL3; IL28B; IL28C; IL-28B; IL-28C; IFN-lambda-3; IFN-lambda-4
• Reactivity: Human
• Synonyms: IL-28B; IL-28B (Human) OmniKine ELISA Kit; Interleukin-28B; Cytokine Zcyto22; Interferon lambda-3; Interferon lambda-4; Interleukin-28C; IL-28B elisa kit

• Reactivity: Human
• Specificity: The OmniKine™ Human IL-28B ELISA is capable of recognizing both recombinant and naturally produced Human IL-28B proteins. The antigens listed below were tested at 50 ng/ml and did not exhibit significant cross reactivity or interference.
• Form/Format: 12 x 8-Well Microstrips
• Sequence Length: 200

• Assay Type: Quantitative Sandwich
• Preparation and Storage: Store at 4 degree C for 6 months.

Related Product Information for IL-28B elisa kit

Principle of the Assay: The OmniKine™ Human IL-28B ELISA Kit contains the components necessary for quantitative determination of natural or recombinant Human IL-28B concentrations within any experimental sample including cell lysates, serum and plasma. This particular immunoassay utilizes the quantitative technique of a "Sandwich" Enzyme-Linked Immunosorbent Assay (ELISA) where the target protein (antigen) is bound in a "sandwich" format by the primary capture antibodies coated to each well-bottom and the secondary detection antibodies added subsequently by the investigator. The capture antibodies coated to the bottom of each well are specific for a particular epitope on Human IL-28B while the user-added detection antibodies bind to epitopes on the captured target protein. Amid each step of the procedure, a series of wash steps must be performed to ensure the elimination of non-specific binding between proteins to other proteins or to the solid phase. After incubation and "sandwiching" of the target antigen, a peroxidase enzyme is conjugated to the constant heavy chain of the secondary antibody (either covalently or via Avidin/Streptavidin-Biotin interactions), allowing for a colorimetric reaction to ensue upon substrate addition. When the substrate TMB (3, 3', 5, 5'-Tetramethylbenzidine) is added, the reaction catalyzed by peroxidase yields a blue color that is representative of the antigen concentration. Upon sufficient color development, the reaction can be terminated through addition of Stop Solution (2 N Sulfuric Acid) where the color of the solution will turn yellow. The absorbance of each well can then be read by a spectrophotometer, allowing for generation of a standard curve and subsequent determination of protein concentration.

Background/Introduction: IL-28B is a cytokine with immunomodulatory activity that up-regulates MHC class I antigen expression. Studies have shown that IL-28A displays potent antiviral activity in addition to antitumor activity. Ligand for the heterodimeric class II cytokine receptor is composed of IL10RB and IL28RA. The ligand/receptor complex seems to signal through the Jak-STAT pathway and induce by viral infections or dsRNA. IL-28B encodes a cytokine distantly related to type I interferons and the IL-10 family. This gene, interleukin 28A (IL28A), and interleukin 29 (IL29) are three closely related cytokine genes that form a cytokine gene cluster on a chromosomal region mapped to 19q13. Expression of the cytokines encoded by the three genes can be induced by viral infection. All three cytokines have been shown to interact with a heterodimeric class II cytokine receptor that consists of interleukin 10 receptor, beta (IL10RB), and interleukin 28 receptor, alpha (IL28RA).

NCBI and Uniprot Product Information

• NCBI GI #: 1100961536
• NCBI GeneID: 282617
• NCBI Accession #: NP_001333866.1
• NCBI GenBank Nucleotide #: NM_001346937.1
• UniProt Accession #: Q8IZI9; Q6VN56; Q7Z4J3; Q8IWL6; A2BDE1
• Molecular Weight: 21,706 Da
• NCBI Official Full Name: interferon lambda-3 isoform 1
• NCBI Official Synonym Full Names: interferon lambda 3
• NCBI Official Symbol: IFNL3
• NCBI Official Synonym Symbols: IL28B; IL28C; IL-28B; IL-28C; IFN-lambda-3; IFN-lambda-4
• NCBI Protein Information: interferon lambda-3
• UniProt Protein Name: Interferon lambda-3
• UniProt Gene Name: IFNL3
• UniProt Synonym Gene Names: IL28B; IL28C; ZCYTO22; IFN-lambda-3; IL-28B; IL-28C

NCBI Description

This gene encodes a cytokine distantly related to type I interferons and the IL-10 family. This gene, interleukin 28A (IL28A), and interleukin 29 (IL29) are three closely related cytokine genes that form a cytokine gene cluster on a chromosomal region mapped to 19q13. Expression of the cytokines encoded by the three genes can be induced by viral infection. All three cytokines have been shown to interact with a heterodimeric class II cytokine receptor that consists of interleukin 10 receptor, beta (IL10RB) and interleukin 28 receptor, alpha (IL28RA). [provided by RefSeq, Jul 2008]

Uniprot Description

IL-28B: Cytokine with immunomodulatory activity. Up-regulates MHC class I antigen expression. Displays potent antiviral activity. Also displays antitumor activity. Ligand for the heterodimeric class II cytokine receptor composed of IL10RB and IL28RA. The ligand/receptor complex seems to signal through the Jak-STAT pathway. Belongs to the IL-28/IL-29 family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 19q13.2

Cellular Component: extracellular space; intracellular

Molecular Function: cytokine activity; receptor binding

Biological Process: defense response to virus; innate immune response; JAK-STAT cascade; negative regulation of viral genome replication; positive regulation of immune response; regulation of T cell activation

Disease: Hepatitis C Virus, Susceptibility To