IL-28B / IFN-lambda-3 recombinant protein

Human IL-28B / IFN-lambda-3 Protein (His Tag)

Cat. Num. AAA2545644

• Gene Names: IFNL3; IL28B; IL28C; IL-28B; IL-28C; IFN-lambda-3; IFN-lambda-4
• Purity: > 95 % as determined by SDS-PAGE.
• Synonyms: IL-28B / IFN-lambda-3; Human IL-28B / IFN-lambda-3 Protein (His Tag); IL28B; IL28C; IL-28B / IFN-lambda-3 recombinant protein

• Host: Yeast
• Purity/Purification: > 95 % as determined by SDS-PAGE.
• Form/Format: Lyophilized from sterile PBS, PH 7.4
• Sequence Length: 196

• Application Notes: The recombinant human IFNL3 consists 189 amino acids and predicts a molecular mass of 21.3 kDa.
• Predicted N Terminal: Val 18
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for IL-28B / IFN-lambda-3 recombinant protein

Background: Interleukin-28B (IL-28B) also known as Interferon lambda-3 and IFN-lambda-3, belongs to the type III interferon family of cytokines and are highly similar to IL-29. IL-28B belongs to the newly described interferon lambda (IFNlambda) family of cytokines. IL-28B is a cytokine with immunomodulatory activity. It functions in Up-regulating MHC class I antigen expression. IL-28B displays potent antiviral activity and antitumor activity. This cytokine serves as ligand for the heterodimeric class II cytokine receptor composed of IL10RB and IL28RA. The ligand/receptor complex seems to signal through the Jak-STAT pathway. IL-28B, like IL-12, is capable of robustly enhancing adaptive immunity. Moreover, we describe for the first time how IL-28B reduces regulatory T-cell populations during DNA vaccination, whereas IL-12 increases this cellular subset. We also show that IL-28B, unlike IL-12, is able to increase the percentage of splenic CD8+ T cells in vaccinated animals, and that these cells are more granular and have higher antigen-specific cytolytic degranulation compared with cells taken from animals that received IL-12 as an adjuvant.

Description: A DNA sequence encoding the human IFNL3 (NP_742151.2) (Val18-Val196) was expressed with a polyhistidine tag at the C-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 28144901
• NCBI GeneID: 282617
• NCBI Accession #: NP_742151.2
• NCBI GenBank Nucleotide #: NM_172139.3
• UniProt Accession #: Q8IZI9; Q6VN56; Q7Z4J3; Q8IWL6; A2BDE1
• Molecular Weight: 21,706 Da
• NCBI Official Full Name: interferon lambda-3 isoform 2
• NCBI Official Synonym Full Names: interferon lambda 3
• NCBI Official Symbol: IFNL3
• NCBI Official Synonym Symbols: IL28B; IL28C; IL-28B; IL-28C; IFN-lambda-3; IFN-lambda-4
• NCBI Protein Information: interferon lambda-3
• UniProt Protein Name: Interferon lambda-3
• UniProt Gene Name: IFNL3
• UniProt Synonym Gene Names: IL28B; IL28C; ZCYTO22; IFN-lambda-3; IL-28B; IL-28C

NCBI Description

This gene encodes a cytokine distantly related to type I interferons and the IL-10 family. This gene, interleukin 28A (IL28A), and interleukin 29 (IL29) are three closely related cytokine genes that form a cytokine gene cluster on a chromosomal region mapped to 19q13. Expression of the cytokines encoded by the three genes can be induced by viral infection. All three cytokines have been shown to interact with a heterodimeric class II cytokine receptor that consists of interleukin 10 receptor, beta (IL10RB) and interleukin 28 receptor, alpha (IL28RA). [provided by RefSeq, Jul 2008]

Uniprot Description

IL-28B: Cytokine with immunomodulatory activity. Up-regulates MHC class I antigen expression. Displays potent antiviral activity. Also displays antitumor activity. Ligand for the heterodimeric class II cytokine receptor composed of IL10RB and IL28RA. The ligand/receptor complex seems to signal through the Jak-STAT pathway. Belongs to the IL-28/IL-29 family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 19q13.2

Cellular Component: extracellular region; extracellular space

Molecular Function: receptor binding

Biological Process: defense response to virus; innate immune response; negative regulation of viral genome replication; regulation of T cell activation

Disease: Hepatitis C Virus, Susceptibility To