Human Histone H2A.Z, H2AFZ ELISA Kit | H2AFZ elisa kit

Human Histone H2A.Z, H2AFZ ELISA Kit

Cat. Num. AAA2602744

• Gene Names: H2AFZ; H2AZ; H2A.z; H2A/z; H2A.Z-1
• Reactivity: Human
• Synonyms: Histone H2A.Z; H2AFZ; Human Histone H2A.Z; H2AFZ ELISA Kit; H2AFZ elisa kit

• Reactivity: Human
• Specificity: No cross-reaction with other factors.

• Samples: Human serum, plasma or cell culture supernatant
• Assay Type: Quantitative Sandwich
• Detection Range: 20 ng/mL-0.312 ng/mL
• Sensitivity: Up to 0.06 ng/mL
• Intra-assay Precision: <= 8%
• Inter-assay Precision: <= 12%
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

()

Related Product Information for H2AFZ elisa kit

Principle of the Assay: As mentioned above, this kit utilizes the Double Antibody Sandwich ELISA technique. The pre-coated antibody is an anti-Human H2AFZ monoclonal antibody, while the detection antibody is a biotinylated polyclonal antibody. Samples and biotinylated antibodies are added into ELISA plate wells and washed out with PBS or TBS after their respective additions to the wells. Then Avidin-peroxidase conjugates are added to the wells in after. TMB substrate is used for coloration after the enzyme conjugate has already been thoroughly washed out of the wells by PBS or TBS. TMB reacts to form a blue product from the peroxidase activity, and finally turns to yellow after addition of the stop solution (Color Reagent C). The color intensity and quantity of target analyte in the sample are positively correlated.

NCBI and Uniprot Product Information

• NCBI GI #: 4504255
• NCBI GeneID: 3015
• NCBI Accession #: NP_002097.1
• NCBI GenBank Nucleotide #: NM_002106.3
• UniProt Accession #: P0C0S5; P17317; Q6I9U0; B2RD56
• Molecular Weight: 13,553 Da
• NCBI Official Full Name: histone H2A.Z
• NCBI Official Synonym Full Names: H2A histone family, member Z
• NCBI Official Symbol: H2AFZ
• NCBI Official Synonym Symbols: H2AZ; H2A.z; H2A/z; H2A.Z-1
• NCBI Protein Information: histone H2A.Z; H2AZ histone
• UniProt Protein Name: Histone H2A.Z
• UniProt Gene Name: H2AFZ
• UniProt Synonym Gene Names: H2AZ; H2A/z
• UniProt Entry Name: H2AZ_HUMAN

NCBI Description

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene encodes a replication-independent member of the histone H2A family that is distinct from other members of the family. Studies in mice have shown that this particular histone is required for embryonic development and indicate that lack of functional histone H2A leads to embryonic lethality. [provided by RefSeq, Jul 2008]

Uniprot Description

H2AZ: Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. May be involved in the formation of constitutive heterochromatin. May be required for chromosome segregation during cell division. The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA. H2A or its variant H2AFZ forms an heterodimer with H2B. H2AFZ interacts with INCENP. Belongs to the histone H2A family.

Protein type: DNA-binding

Chromosomal Location of Human Ortholog: 4q24

Cellular Component: Barr body; nuclear heterochromatin; nucleosome; nucleus

Molecular Function: protein binding; nucleosomal DNA binding; protein heterodimerization activity; chromatin DNA binding

Biological Process: positive regulation of transcription from RNA polymerase II promoter