Rabbit anti-Human NKX3-1 Polyclonal Antibody | anti-NKX3-1 antibody

Rabbit Polyclonal to Human NKX3-1

Cat. Num. AAA248051

• Gene Names: NKX3-1; NKX3; BAPX2; NKX3A; NKX3.1
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot, Flow Cytometry, Functional Assay
• Purity: Immunoaffinity Purified
• Synonyms: NKX3-1; Polyclonal Antibody; Rabbit Polyclonal to Human NKX3-1; Anti-NKX3-1 Antibody (aa118-145) IHC-plus; BAPX2; NK homeobox; family 3; A; NK3 homeobox 1; NKX3; NKX3.1; Homeobox protein Nkx-3.1; NKX3A; Human NKX3-1; anti-NKX3-1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: Human NKX3-1
• Purity/Purification: Immunoaffinity Purified
• Form/Format: PBS, 0.09% sodium azide
• Sequence Length: 234

• Applicable Applications for anti-NKX3-1 antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB), Flow Cytometry (FC/FACS)
• Application Notes: Flo (1:10 - 1:50), IHC-P (1:100), WB (1:1000)
• Target Species: Human
• Immunogen Description: KLH-conjugated synthetic peptide from internal region of human NKX3-1.
• Immunogen Type: Synthetic peptide
• Immunogen: NKX3-1 antibody was raised against kLH-conjugated synthetic peptide from internal region of human NKX3-1.
• Antigen Modification: aa118-145
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Immunohistochemistry (IHC)

(Anti-NKX3-1 antibody IHC staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.)

Flow Cytometry (FC/FACS)

(NKX3-1 Antibody (Center) flow cytometry analysis of MDA-MB231 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Western Blot (WB)

(Western blot of NKX3-1 Antibody (Center) in MCF-7 cell line lysates (35 ug/lane). NKX3-1 (arrow) was detected using the purified Pab.)

NCBI and Uniprot Product Information

• NCBI GI #: 19923352
• NCBI GeneID: 4824
• NCBI Accession #: NP_006158.2
• NCBI GenBank Nucleotide #: NM_006167.3
• UniProt Accession #: Q99801; O15465; Q9H2P4; Q9H2P5; Q9H2P6; Q9H2P7; Q9HBG0
• Molecular Weight: 21,626 Da
• NCBI Official Full Name: homeobox protein Nkx-3.1 isoform 1
• NCBI Official Synonym Full Names: NK3 homeobox 1
• NCBI Official Symbol: NKX3-1
• NCBI Official Synonym Symbols: NKX3; BAPX2; NKX3A; NKX3.1
• NCBI Protein Information: homeobox protein Nkx-3.1; NK homeobox, family 3, A; homeobox protein NK-3 homolog A; NK3 transcription factor homolog A; NK3 transcription factor related, locus 1
• UniProt Protein Name: Homeobox protein Nkx-3.1
• Protein Family: Homeobox protein
• UniProt Gene Name: NKX3-1
• UniProt Synonym Gene Names: NKX3.1; NKX3A
• UniProt Entry Name: NKX31_HUMAN

NCBI Description

This gene encodes a homeobox-containing transcription factor. This transcription factor functions as a negative regulator of epithelial cell growth in prostate tissue. Aberrant expression of this gene is associated with prostate tumor progression. Alternate splicing results in multiple transcript variants of this gene. [provided by RefSeq, Jan 2012]

Uniprot Description

NKX3-1: Transcription factor, which binds preferentially the consensus sequence 5'-TAAGT[AG]-3' and can behave as a transcriptional repressor. Play an important role in normal prostate development, regulating proliferation of glandular epithelium and in the formation of ducts in prostate. Act as a tumor suppressor controlling prostate carcinogenesis, as shown by the ability to inhibit proliferation and invasion activities of PC-3 prostate cancer cells. Interacts with serum response factor (SRF). Interacts with SPDEF. Interacts with WDR77. Interacts with TOPORS which polyubiquitinates NKX3-1 and induces its proteasomal degradation. By androgens and, in the LNCAP cell line, by estrogens. Androgenic control may be lost in prostate cancer cells during tumor progression from an androgen-dependent to an androgen- independent phase. Highly expressed in the prostate and, at a lower level, in the testis. Belongs to the NK-3 homeobox family. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding; Transcription factor; Tumor suppressor

Chromosomal Location of Human Ortholog: 8p21.2

Cellular Component: intracellular; nucleus

Molecular Function: androgen receptor activity; protein binding; estrogen receptor activity; protein self-association; sequence-specific DNA binding; histone deacetylase binding; estrogen receptor binding; caspase activator activity; transcription factor binding; protein kinase activator activity; transcription factor activity

Biological Process: transcription from RNA polymerase II promoter; salivary gland development; positive regulation of transcription, DNA-dependent; multicellular organismal development; heart development; negative regulation of insulin-like growth factor receptor signaling pathway; positive regulation of caspase activity; positive regulation of mitotic cell cycle; negative regulation of cell proliferation; regulation of transcription, DNA-dependent; positive regulation of cell proliferation; protein kinase B signaling cascade; negative regulation of mitotic cell cycle; negative regulation of epithelial cell proliferation; caspase activation; somitogenesis; pharyngeal system development; male gonad development; response to testosterone stimulus; positive regulation of phosphoinositide 3-kinase cascade; branching morphogenesis of a tube; positive regulation of protein kinase activity; positive regulation of cell division; androgen receptor signaling pathway; steroid hormone mediated signaling; positive regulation of transcription from RNA polymerase II promoter; positive regulation of protein amino acid phosphorylation; negative regulation of transcription, DNA-dependent; metanephros development