Human Histamine Receptor H1 (HRH1) ELISA Kit | HRH1 elisa kit

Human Histamine Receptor H1 (HRH1) ELISA Kit

Cat. Num. AAA2706001

• Gene Names: Hrh1; H1R; Hir; Bphs; HH1R
• Reactivity: Human
• Synonyms: Histamine Receptor H1 (HRH1); Human Histamine Receptor H1 (HRH1) ELISA Kit; H1-R; hisH1; HRH1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Histamine Receptor H1 (HRH1).; No significant cross-reactivity or interference between Histamine Receptor H1 (HRH1) and analogues was observed.
• Sequence Length: 488

• Assay Type: Double-antibody Sandwich
• Samples: Tissue Homogenates, Cell Lysates and Other Biological Fluids
• Detection Range: 0.312-20ng/mL
• Sensitivity: < 0.114ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Histamine Receptor H1 (HRH1) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Histamine Receptor H1 (HRH1) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for HRH1 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Histamine Receptor H1 (HRH1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Histamine Receptor H1 (HRH1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Histamine Receptor H1 (HRH1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Histamine Receptor H1 (HRH1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 14626757
• NCBI GeneID: 15465
• UniProt Accession #: P70174; Q91V75; Q91XN0; Q91XN1; Q91XN2; Q91XN3
• Molecular Weight: 55,682 Da
• NCBI Official Full Name: histamine receptor H1
• NCBI Official Synonym Full Names: histamine receptor H1
• NCBI Official Symbol: Hrh1
• NCBI Official Synonym Symbols: H1R; Hir; Bphs; HH1R
• NCBI Protein Information: histamine H1 receptor
• UniProt Protein Name: Histamine H1 receptor
• Protein Family: Histamine H1 receptor
• UniProt Gene Name: Hrh1
• UniProt Synonym Gene Names: Bphs; H1R; HH1R

Uniprot Description

In peripheral tissues, the H1 subclass of histamine receptors mediates the contraction of smooth muscles, increase in capillary permeability due to contraction of terminal venules, and catecholamine release from adrenal medulla, as well as mediating neurotransmission in the central nervous system. Involved in circadian rhythm of locomotor activity and exploratory behavior. Also involved in responsiveness to pertussis toxin through its control of susceptibility to histamine hypersensitivity and enhancement of antigen-specific delayed-type hypersensitivity responses.