Typical Testing Data/Standard Curve (for reference only)
Rat Histamine Receptor H1 (HRH1) ELISA Kit | HRH1 elisa kit
Rat Histamine Receptor H1 (HRH1) ELISA Kit
Gene Names
Hrh1; H1R; Hir; Bphs; HH1R
Reactivity
Rat
Synonyms
Histamine Receptor H1 (HRH1); Rat Histamine Receptor H1 (HRH1) ELISA Kit; H1-R; hisH1; HRH1 elisa kit
Reactivity
Rat
Specificity
This assay has high sensitivity and excellent specificity for detection of Histamine Receptor H1 (HRH1).
No significant cross-reactivity or interference between Histamine Receptor H1 (HRH1) and analogues was observed.
No significant cross-reactivity or interference between Histamine Receptor H1 (HRH1) and analogues was observed.
Sequence Length
488
Assay Type
Double-antibody Sandwich
Samples
Tissue homogenates and Other Biological Fluids
Detection Range
0.156-10ng/mL
Sensitivity
< 0.067ng/mL
Application
Enzyme-linked immunosorbent assay for Antigen Detection.
Intra-assay Precision (Precision within an assay)
3 samples with low, middle and high level Histamine Receptor H1 (HRH1) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays)
3 samples with low, middle and high level Histamine Receptor H1 (HRH1) were tested on 3 different plates, 8 replicates in each plate.
CV(%) =
SD/meanX100
Intra-Assay
CV<10%
Inter-Assay
CV<12%
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for HRH1 elisa kit
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Histamine Receptor H1 (HRH1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Histamine Receptor H1 (HRH1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Histamine Receptor H1 (HRH1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Histamine Receptor H1 (HRH1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Product Categories/Family for HRH1 elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
55,682 Da
NCBI Official Full Name
histamine receptor H1
NCBI Official Synonym Full Names
histamine receptor H1
NCBI Official Symbol
Hrh1
NCBI Official Synonym Symbols
H1R; Hir; Bphs; HH1R
NCBI Protein Information
histamine H1 receptor
UniProt Protein Name
Histamine H1 receptor
Protein Family
UniProt Gene Name
Hrh1
UniProt Synonym Gene Names
Bphs; H1R; HH1R
UniProt Entry Name
HRH1_MOUSE
Uniprot Description
H1R: In peripheral tissues, the H1 subclass of histamine receptors mediates the contraction of smooth muscles, increase in capillary permeability due to contraction of terminal venules, and catecholamine release from adrenal medulla, as well as mediating neurotransmission in the central nervous system. Belongs to the G-protein coupled receptor 1 family.
Protein type: GPCR, family 1; Membrane protein, integral; Membrane protein, multi-pass; Receptor, GPCR
Cellular Component: nucleoplasm; membrane; cytoplasm; integral to membrane; plasma membrane
Molecular Function: G-protein coupled receptor activity; signal transducer activity; histamine binding; calcium ion transmembrane transporter activity; histamine receptor activity
Biological Process: G-protein coupled receptor protein signaling pathway; synaptic transmission; positive regulation of nitric oxide biosynthetic process; rhythmic process; positive regulation of vasoconstriction; eosinophil chemotaxis; regulation of synaptic plasticity; visual learning; regulation of vascular permeability; signal transduction; memory
Research Articles on HRH1
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Product Notes
The Rat HRH1 hrh1 (Catalog #AAA2019968) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2019968 ELISA Kit recognizes Rat HRH1. It is sometimes possible for the material contained within the vial of "Histamine Receptor H1 (HRH1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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