Western Blot (WB)
(The anti-MAGE-A4 Pab is used in Western blot to detect MAGE-A4 in A375 cell lysate.)
Rabbit anti-Human MAGEA4 Polyclonal Antibody | anti-MAGEA4 antibody
MAGEA4 Antibody (N-term)
Western Blot (WB)
(The anti-MAGE-A4 Pab is used in Western blot to detect MAGE-A4 in A375 cell lysate.)
Western Blot (WB)
(The anti-MAGE-A4 Pab is used in Western blot to detect MAGE-A4 in A375 cell lysate.)
Immunohistochemistry (IHC)
(Immunostaining of paraformldehyde-fixed and paraffin-embedded human breast carcinoma with MAGE-A4 antibody (1:50 dilution), followed by reacting with biotin-conjugated secondary antibody, ABC solution and developing with DAB. Cancerous cells show nuclear staining of MAGE-A4. Magnification: 40X. Data courtesy of Dr. Mi Hou, Karolinska Institutet and University Hospital, Sweden.)
Immunohistochemistry (IHC)
(Immunostaining of paraformldehyde-fixed and paraffin-embedded human breast carcinoma with MAGE-A4 antibody (1:50 dilution), followed by reacting with biotin-conjugated secondary antibody, ABC solution and developing with DAB. Cancerous cells show nuclear staining of MAGE-A4. Magnification: 40X. Data courtesy of Dr. Mi Hou, Karolinska Institutet and University Hospital, Sweden.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with MAGEA4 antibody (N-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with MAGEA4 antibody (N-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
hereditary disorders, such as dyskeratosis congenita.
Resnick, M.B., et al., Int. J. Cancer 101(2):190-195 (2002).
Imai, Y., et al., Gene 160(2):287-290 (1995).
Rogner, U.C., et al., Genomics 29(3):725-731 (1995).
De Plaen, E., et al., Immunogenetics 40(5):360-369 (1994).
NCBI and Uniprot Product Information
NCBI Description
This gene is a member of the MAGEA gene family. The members of this family encode proteins with 50 to 80% sequence identity to each other. The promoters and first exons of the MAGEA genes show considerable variability, suggesting that the existence of this gene family enables the same function to be expressed under different transcriptional controls. The MAGEA genes are clustered at chromosomal location Xq28. They have been implicated in some hereditary disorders, such as dyskeratosis congenita. At least four variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]
Uniprot Description
MAGE-A4: a member of the MAGE (melanoma-associated antigen) family of Cancer-Testis Antigens (CTAs). May play a role in embryonal development and tumor transformation or aspects of tumor progression. CTAs were once thought to be silenced in most normal adult tissues, with limited expression in fetal, placental, testis, and ovary cells. These proteins are now known to be aberrantly expressed in various cancers and many are capable of eliciting humoral and cellular immune responses. The MAGE family of proteins, encompassing 12 highly homologous genes clustered at Xq26-28, are tumor-specific antigens that can be recognized by autologous cytolytic T lymphocytes. MAGE proteins are characterized by the presence of a conserved domain (MHD, MAGE Homology Domain). MAGE-A4 expression is high in ovarian serous carcinoma, various squamous carcinomas, testicular germ cell tumors, oesophageal mucosal melanoma, and triple-negative breast cancers. Spontaneous humoral and cellular immune responses against MAGE-A4 have been detected in cancer patients. MAGE family proteins bind to and activate RING E3 ubiquitin ligases.
Protein type: Cancer Testis Antigen (CTA)
Chromosomal Location of Human Ortholog: Xq28
Molecular Function: protein binding
Research Articles on MAGEA4
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Product Notes
The MAGEA4 magea4 (Catalog #AAA9200812) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 9-38. The MAGEA4 Antibody (N-term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's MAGEA4 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC). WB~~1:1000. Researchers should empirically determine the suitability of the MAGEA4 magea4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MAGEA4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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