Human High Mobility Group Box Protein 3 (HMGB3) ELISA Kit | HMGB3 elisa kit

Human High Mobility Group Box Protein 3 (HMGB3) ELISA Kit

Cat. Num. AAA2098046

• Gene Names: HMGB3; HMG4; HMG-4; HMG2A; HMG-2a
• Reactivity: Human
• Synonyms: High Mobility Group Box Protein 3 (HMGB3); Human High Mobility Group Box Protein 3 (HMGB3) ELISA Kit; HMG2A; HMG4; High mobility group protein 4; HMGB3 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of High Mobility Group Box Protein 3 (HMGB3).; No significant cross-reactivity or interference between High Mobility Group Box Protein 3 (HMGB3) and analogues was observed.
• Sequence Length: 200

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma and Other Biological Fluids
• Detection Range: 0.78-50ng/mL
• Sensitivity: < 0.27ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level High Mobility Group Box Protein 3 (HMGB3) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level High Mobility Group Box Protein 3 (HMGB3) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for HMGB3 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to High Mobility Group Box Protein 3 (HMGB3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to High Mobility Group Box Protein 3 (HMGB3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain High Mobility Group Box Protein 3 (HMGB3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of High Mobility Group Box Protein 3 (HMGB3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for HMGB3 elisa kit

Infection immunity

NCBI and Uniprot Product Information

• NCBI GI #: 669033279
• NCBI GeneID: 3149
• NCBI Accession #: NP_001288157.1
• NCBI GenBank Nucleotide #: NM_001301228.1
• UniProt Accession #: O15347; O95556; Q6NS40
• Molecular Weight: 22,980 Da
• NCBI Official Full Name: high mobility group protein B3 isoform a
• NCBI Official Synonym Full Names: high mobility group box 3
• NCBI Official Symbol: HMGB3
• NCBI Official Synonym Symbols: HMG4; HMG-4; HMG2A; HMG-2a
• NCBI Protein Information: high mobility group protein B3
• UniProt Protein Name: High mobility group protein B3
• Protein Family: High mobility group protein
• UniProt Gene Name: HMGB3
• UniProt Synonym Gene Names: HMG2A; HMG4; HMG-2a; HMG-4

NCBI Description

This gene encodes a member of a family of proteins containing one or more high mobility group DNA-binding motifs. The encoded protein plays an important role in maintaining stem cell populations, and may be aberrantly expressed in tumor cells. A mutation in this gene was associated with microphthalmia, syndromic 13. There are numerous pseudogenes of this gene on multiple chromosomes. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2014]

Uniprot Description

Multifunctional protein with various roles in different cellular compartments. May act in a redox sensitive manner. Associates with chromatin and binds DNA with a preference to non-canonical DNA structures such as single-stranded DNA. Can bent DNA and enhance DNA flexibility by looping thus providing a mechanism to promote activities on various gene promoters (). Proposed to be involved in the innate immune response to nucleic acids by acting as a cytoplasmic promiscuous immunogenic DNA/RNA sensor (). Negatively regulates B-cell and myeloid cell differentiation. In hematopoietic stem cells may regulate the balance between self-renewal and differentiation. Involved in negative regulation of canonical Wnt signaling ().