Human Heart-type Fatty Acid Binding Protein (H-FABP) ELISA Kit | H-FABP elisa kit

Human Heart-type Fatty Acid Binding Protein (H-FABP) High Sensitive ELISA Kit

Cat. Num. AAA2708056

• Gene Names: FABP3; MDGI; FABP11; H-FABP; M-FABP; O-FABP
• Reactivity: Human
• Applications: ELISA
• Synonyms: Heart-type Fatty Acid Binding Protein (H-FABP); Human Heart-type Fatty Acid Binding Protein (H-FABP) High Sensitive ELISA Kit; Heart-type Fatty Acid Binding Protein; MDGI; FABP11; H-FABP; O-FABP; Hfabp; FABP3; Fatty Acid Binding Protein 3; Muscle And Heart; Mammary Derived Growth Inhibitor; Fatty Acid Binding Protein 11; Muscle fatty acid-binding protein; Heart-ty; H-FABP elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of High Sensitive Heart-type Fatty Acid Binding Protein (H-FABP).; No significant cross-reactivity or interference between High Sensitive Heart-type Fatty Acid Binding Protein (H-FABP) and analogues was observed.
• Sequence Length: 133

• Applicable Applications for H-FABP elisa kit: ELISA (EIA)
• Assay Type: Double-antibody Sandwich
• Samples: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Detection Range: 62.5-4,000pg/mL
• Sensitivity: The minimum detectable dose of this kit is typically less than 24.7pg/mL
• Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level High Sensitive Heart-type Fatty Acid Binding Protein (H-FABP) were tested 20 times on one plate, respectively.; Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level High Sensitive Heart-type Fatty Acid Binding Protein (H-FABP) were tested on 3 different plates, 8 replicates in each plate.; CV(%) = SD/meanX100; Intra-Assay: CV<10%; Inter-Assay: CV<12%
• Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to High Sensitive Heart-type Fatty Acid Binding Protein (H-FABP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to High Sensitive Heart-type Fatty Acid Binding Protein (H-FABP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain High Sensitive Heart-type Fatty Acid Binding Protein (H-FABP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of High Sensitive Heart-type Fatty Acid Binding Protein (H-FABP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

NCBI and Uniprot Product Information

• NCBI GI #: 119802
• NCBI GeneID: 2170
• UniProt Accession #: P05413
• NCBI Official Full Name: Fatty acid-binding protein, heart
• NCBI Official Synonym Full Names: fatty acid binding protein 3
• NCBI Official Symbol: FABP3
• NCBI Official Synonym Symbols: MDGI; FABP11; H-FABP; M-FABP; O-FABP
• NCBI Protein Information: fatty acid-binding protein, heart
• UniProt Protein Name: Fatty acid-binding protein, heart
• UniProt Gene Name: FABP3
• UniProt Synonym Gene Names: FABP11; MDGI; H-FABP; MDGI; M-FABP
• UniProt Entry Name: FABPH_HUMAN

NCBI Description

The intracellular fatty acid-binding proteins (FABPs) belongs to a multigene family. FABPs are divided into at least three distinct types, namely the hepatic-, intestinal- and cardiac-type. They form 14-15 kDa proteins and are thought to participate in the uptake, intracellular metabolism and/or transport of long-chain fatty acids. They may also be responsible in the modulation of cell growth and proliferation. Fatty acid-binding protein 3 gene contains four exons and its function is to arrest growth of mammary epithelial cells. This gene is a candidate tumor suppressor gene for human breast cancer. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2016]

Uniprot Description

FABP3: FABP are thought to play a role in the intracellular transport of long-chain fatty acids and their acyl-CoA esters. Belongs to the calycin superfamily. Fatty-acid binding protein (FABP) family.

Protein type: Lipid-binding

Chromosomal Location of Human Ortholog: 1p33-p32

Cellular Component: extracellular space; sarcoplasm; cytosol

Molecular Function: protein binding; icosatetraenoic acid binding; cytoskeletal protein binding; long-chain fatty acid transporter activity

Biological Process: response to drug; negative regulation of cell proliferation; cholesterol homeostasis; regulation of fatty acid oxidation; fatty acid metabolic process; response to insulin stimulus; phospholipid homeostasis