Human heart fatty acid binding protein, h-FABP ELISA Kit | H-FABP elisa kit

Human heart fatty acid binding protein, h-FABP ELISA Kit

Cat. Num. AAA724660

• Gene Names: FABP3; MDGI; FABP11; H-FABP; O-FABP
• Reactivity: Human
• Synonyms: heart fatty acid binding protein; h-FABP; Human heart fatty acid binding protein; h-FABP ELISA Kit; Human heart fatty acid binding protein ELISA Kit; h-FABP ELISA Kit (Human); H-FABP elisa kit

• Reactivity: Human

• Assay Type: Sandwich
• Preparation and Storage: Store all reagents at 2-8 degree C

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for H-FABP elisa kit

Intended Uses: This H-FABP ELISA kit is intended Laboratory for research use only.The Stop Solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration ofH-FABP in the sample, thisH-FABP ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versusH-FABP concentration. The concentration ofH-FABP in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Principle of the Assay: This H-FABP enzyme linked immunosorbent assay applies a technique called a quantitative sandwich immunoassay. The microtiter plate provided in this kit has been pre-coated with a monoclonal antibody specific forH-FABP. Standards or samples are then added to the microtiter plate wells andH-FABP if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount ofH-FABP present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific forH-FABP are added to each well to "sandwich" theH-FABP immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, A and B substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period.Only those wells that containH-FABP and enzyme-conjugated antibody will exhibit a change in colour. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the colour change is measured spectrophotometrically at a wavelength of 450 nm.

Product Categories/Family for H-FABP elisa kit

Human ELISA Kit

NCBI and Uniprot Product Information

• NCBI GI #: 458862
• NCBI GeneID: 2170
• UniProt Accession #: P05413; Q5VV93; Q99957; B2RAB6; Q6IBD7
• Molecular Weight: 14,858 Da
• NCBI Official Full Name: heart fatty acid binding protein
• NCBI Official Synonym Full Names: fatty acid binding protein 3, muscle and heart (mammary-derived growth inhibitor)
• NCBI Official Symbol: FABP3
• NCBI Official Synonym Symbols: MDGI; FABP11; H-FABP; O-FABP
• NCBI Protein Information: fatty acid-binding protein, heart; M-FABP; OTTHUMP00000003898; fatty acid binding protein 11; mammary-derived growth inhibitor; muscle fatty acid-binding protein; Fatty acid-binding protein 3, muscle; heart-type fatty acid-binding protein
• UniProt Protein Name: Fatty acid-binding protein, heart
• UniProt Gene Name: FABP3
• UniProt Synonym Gene Names: FABP11; MDGI
• UniProt Entry Name: FABPH_HUMAN

NCBI Description

The intracellular fatty acid-binding proteins (FABPs) belongs to a multigene family. FABPs are divided into at least three distinct types, namely the hepatic-, intestinal- and cardiac-type. They form 14-15 kDa proteins and are thought to participate in the uptake, intracellular metabolism and/or transport of long-chain fatty acids. They may also be responsible in the modulation of cell growth and proliferation. Fatty acid-binding protein 3 gene contains four exons and its function is to arrest growth of mammary epithelial cells. This gene is a candidate tumor suppressor gene for human breast cancer. [provided by RefSeq]

Uniprot Description

Function: FABP are thought to play a role in the intracellular transport of long-chain fatty acids and their acyl-CoA esters.

Subcellular location: Cytoplasm.

Domain: Forms a beta-barrel structure that accommodates the hydrophobic ligand in its interior.

Sequence similarities: Belongs to the calycin superfamily. Fatty-acid binding protein (FABP) family.