Loading...

Skip to main content

Call us on + 1 (800) 604-9114 for more information about our products

Looking for specific datasheet Manual/COA/MSDS?
Request a Manual/COA/MSDS

Interested to get a quote about our products?
Request a Quote

Western Blot (WB) (Western blot analysis GDNF using NIH-3T3 whole cell lysates)

Rabbit anti-Human GDNF Polyclonal Antibody | anti-GDNF antibody

GDNF Antibody

Gene Names
GDNF; ATF; ATF1; ATF2; HSCR3; HFB1-GDNF
Reactivity
Human
Applications
Western Blot
Synonyms
GDNF; Polyclonal Antibody; GDNF Antibody; HSCR3; HFB1-GDNF; ATF; HGDNF; anti-GDNF antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Specificity
GDNF Antibody detects endogenous levels of total GDNF
Form/Format
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1.0mg/mL (varies by lot)
Sequence Length
211
Applicable Applications for anti-GDNF antibody
Western Blot (WB)
Application Notes
Western Blot: 1:1000-3000
Immunogen
A synthesized peptide derived from human GDNF
Immunogen Type
Peptide
Preparation and Storage
Store at -20 degree C

Western Blot (WB)

(Western blot analysis GDNF using NIH-3T3 whole cell lysates)

Western Blot (WB) (Western blot analysis GDNF using NIH-3T3 whole cell lysates)
Product Categories/Family for anti-GDNF antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
18,123 Da
NCBI Official Full Name
glial cell line-derived neurotrophic factor isoform 1 preproprotein
NCBI Official Synonym Full Names
glial cell derived neurotrophic factor
NCBI Official Symbol
GDNF
NCBI Official Synonym Symbols
ATF; ATF1; ATF2; HSCR3; HFB1-GDNF
NCBI Protein Information
glial cell line-derived neurotrophic factor
UniProt Protein Name
Glial cell line-derived neurotrophic factor
Protein Family
UniProt Gene Name
GDNF
UniProt Synonym Gene Names
hGDNF; ATF
UniProt Entry Name
GDNF_HUMAN

NCBI Description

This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate each subunit of the disulfide-linked homodimer. The recombinant form of this protein, a highly conserved neurotrophic factor, was shown to promote the survival and differentiation of dopaminergic neurons in culture, and was able to prevent apoptosis of motor neurons induced by axotomy. This protein is a ligand for the product of the RET (rearranged during transfection) protooncogene. Mutations in this gene may be associated with Hirschsprung disease and Tourette syndrome. This gene encodes multiple protein isoforms that may undergo similar proteolytic processing. [provided by RefSeq, Aug 2016]

Uniprot Description

GDNF: Neurotrophic factor that enhances survival and morphological differentiation of dopaminergic neurons and increases their high-affinity dopamine uptake. Defects in GDNF may be a cause of Hirschsprung disease type 3 (HSCR3). In association with mutations of RET gene, defects in GDNF may be involved in Hirschsprung disease. This genetic disorder of neural crest development is characterized by the absence of intramural ganglion cells in the hindgut, often resulting in intestinal obstruction. Defects in GDNF are a cause of congenital central hypoventilation syndrome (CCHS); also known as congenital failure of autonomic control or Ondine curse. CCHS is a rare disorder characterized by abnormal control of respiration in the absence of neuromuscular or lung disease, or an identifiable brain stem lesion. A deficiency in autonomic control of respiration results in inadequate or negligible ventilatory and arousal responses to hypercapnia and hypoxemia. Belongs to the TGF-beta family. GDNF subfamily. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 5p13.1-p12

Cellular Component: extracellular region

Molecular Function: protein binding; protein homodimerization activity; Ras guanyl-nucleotide exchange factor activity; receptor binding

Biological Process: adult locomotory behavior; enteric nervous system development; MAPKKK cascade; metanephros development; mRNA stabilization; negative regulation of apoptosis; negative regulation of neuron apoptosis; nervous system development; neural crest cell migration; neurite development; peristalsis; positive regulation of cell differentiation; positive regulation of cell proliferation; positive regulation of dopamine secretion; positive regulation of monooxygenase activity; positive regulation of transcription from RNA polymerase II promoter; postganglionic parasympathetic nervous system development; regulation of dopamine uptake; regulation of gene expression; signal transduction; sympathetic nervous system development; ureteric bud branching

Disease: Central Hypoventilation Syndrome, Congenital; Hirschsprung Disease, Susceptibility To, 3; Pheochromocytoma

Research Articles on GDNF

Similar Products

Product Notes

The GDNF gdnf (Catalog #AAA9418514) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The GDNF Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's GDNF can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Western Blot: 1:1000-3000. Researchers should empirically determine the suitability of the GDNF gdnf for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GDNF, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

Item has been added to Shopping Cart

If you are ready to order, navigate to Shopping Cart and get ready to checkout.

Looking for a specific manual?
Request a Manual

Request more Information

Please complete the form below and a representative will contact you as soon as possible.

Request a Manual

Please complete the form below and a representative will contact you as soon as possible.

Request a Quote

Please complete the form below and a representative will contact you as soon as possible.