Human Growth Hormone Secretagogue Receptor (GHSR) ELISA Kit | GHSR elisa kit

Human Growth Hormone Secretagogue Receptor (GHSR) ELISA Kit

Cat. Num. AAA2704504

• Gene Names: GHSR; GHDP
• Reactivity: Human
• Synonyms: Growth Hormone Secretagogue Receptor (GHSR); Human Growth Hormone Secretagogue Receptor (GHSR) ELISA Kit; GHRP; GH-releasing peptide receptor; Ghrelin receptor; GHSR elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Growth Hormone Secretagogue Receptor (GHSR).; No significant cross-reactivity or interference between Growth Hormone Secretagogue Receptor (GHSR) and analogues was observed.
• Sequence Length: 289

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma, Tissue Homogenates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.055ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Growth Hormone Secretagogue Receptor (GHSR) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Growth Hormone Secretagogue Receptor (GHSR) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for GHSR elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Growth Hormone Secretagogue Receptor (GHSR). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Growth Hormone Secretagogue Receptor (GHSR). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Growth Hormone Secretagogue Receptor (GHSR), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Growth Hormone Secretagogue Receptor (GHSR) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 4039146
• NCBI GeneID: 2693
• UniProt Accession #: Q92847; Q14D12; Q6ISR8; Q92848; Q96RJ7
• Molecular Weight: 32,153 Da
• NCBI Official Full Name: growth hormone secretagogue receptor, partial
• NCBI Official Synonym Full Names: growth hormone secretagogue receptor
• NCBI Official Symbol: GHSR
• NCBI Official Synonym Symbols: GHDP
• NCBI Protein Information: growth hormone secretagogue receptor type 1
• UniProt Protein Name: Growth hormone secretagogue receptor type 1
• Protein Family: Growth hormone secretagogue receptor
• UniProt Gene Name: GHSR
• UniProt Synonym Gene Names: GHS-R; GHRP

NCBI Description

This gene encodes a member of the G-protein coupled receptor family. The encoded protein may play a role in energy homeostasis and regulation of body weight. Two identified transcript variants are expressed in several tissues and are evolutionary conserved in fish and swine. One transcript, 1a, excises an intron and encodes the functional protein; this protein is the receptor for the Ghrelin ligand and defines a neuroendocrine pathway for growth hormone release. The second transcript (1b) retains the intron and does not function as a receptor for Ghrelin; however, it may function to attenuate activity of isoform 1a. Mutations in this gene are associated with autosomal idiopathic short stature.[provided by RefSeq, Apr 2010]

Uniprot Description

Receptor for ghrelin, coupled to G-alpha-11 proteins. Stimulates growth hormone secretion. Binds also other growth hormone releasing peptides (GHRP) (e.g. Met-enkephalin and GHRP-6) as well as non-peptide, low molecular weight secretagogues (e.g. L-692,429, MK-0677, adenosine).