Western Blot (WB)
(Western Blot analysis of Human stomach tissue using NF-L Monoclonal Antibody at dilution of 1:1000)
Mouse Neurofilament Monoclonal Antibody | anti-NF-L antibody
Neurofilament Monoclonal Antibody
Gene Names
NEFL; NFL; NF-L; NF68; CMT1F; CMT2E; CMTDIG; PPP1R110
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, ELISA
Purity
Protein G purification
Synonyms
Neurofilament; Monoclonal Antibody; Neurofilament Monoclonal Antibody; 68 kDa neurofilament protein; NEFL; Neurofilament L; NF68; NFL; NF-L; anti-NF-L antibody
Host
Mouse
Reactivity
Human, Mouse, Rat
Clonality
Monoclonal
Isotype
IgG1
Purity/Purification
Protein G purification
Form/Format
PBS with 0.02% sodium azide, 50% glycerol, PH7.3
Sequence Length
543
Applicable Applications for anti-NF-L antibody
Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
Application Notes
WB: 1:500-1:5000
IHC: 1:20-1:200
IHC: 1:20-1:200
Immunogen
Fusion protein of NF-L
Preparation and Storage
Store at -20 degree C. Avoid freeze / thaw cycles.
Western Blot (WB)
(Western Blot analysis of Human stomach tissue using NF-L Monoclonal Antibody at dilution of 1:1000)
Western Blot (WB)
(Western Blot analysis of Human stomach tissue using NF-L Monoclonal Antibody at dilution of 1:1000)
Related Product Information for anti-NF-L antibody
NEFL, also named as NF68 and NFL, belongs to the intermediate filament family. Neurofilaments are the 10nm intermediate filaments found specifically in neurons. They are a major component of the cell's cytoskeleton, and provide support for normal axonal radial growth. Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. The names given to the three major neurofilament subunits are based upon the apparent molecular weight of the mammalian subunits on SDS-PAGE:NF-L, 65-68kd; NF-M, 145-160kd and NF-H, 200-220kd. This antibody is specific to NEFL. It has a very weak reaction to NEFM.
Product Categories/Family for anti-NF-L antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Calculated: 543aa, 62kDa
Observed: 65kDa
Observed: 65kDa
NCBI Official Full Name
neurofilament light polypeptide
NCBI Official Synonym Full Names
neurofilament light
NCBI Official Symbol
NEFL
NCBI Official Synonym Symbols
NFL; NF-L; NF68; CMT1F; CMT2E; CMTDIG; PPP1R110
NCBI Protein Information
neurofilament light polypeptide
UniProt Protein Name
Neurofilament light polypeptide
Protein Family
UniProt Gene Name
NEFL
UniProt Synonym Gene Names
NF68; NFL; NF-L
NCBI Description
Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and they functionally maintain the neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the light chain neurofilament protein. Mutations in this gene cause Charcot-Marie-Tooth disease types 1F (CMT1F) and 2E (CMT2E), disorders of the peripheral nervous system that are characterized by distinct neuropathies. A pseudogene has been identified on chromosome Y. [provided by RefSeq, Oct 2008]
Uniprot Description
Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber.
Research Articles on NF-L
Similar Products
Product Notes
The NF-L nefl (Catalog #AAA2541999) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Neurofilament Monoclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Neurofilament can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA). WB: 1:500-1:5000 IHC: 1:20-1:200. Researchers should empirically determine the suitability of the NF-L nefl for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Neurofilament, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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