Human GM-CSF ELISA Kit | GM-CSF elisa kit

Human GM-CSF ELISA Kit

Cat. Num. AAA9711543

• Gene Names: CSF2; CSF; GMCSF
• Reactivity: Human
• Synonyms: GM-CSF; Human GM-CSF ELISA Kit; GMCSF; Colony stimulating factor; Granulocyte Macrophage Colony Stimulating Factor; CSF 2; CSF2; GM-CSF elisa kit

• Reactivity: Human
• Specificity: Human GM-CSF ELISA Kit has high sensitivity and excellent specificity for detection of Human GM-CSF. No significant cross-reactivity or interference between Human GM-CSF and analogues was observed.
• Sequence Length: 144

• Assay Type: Sandwich ELISA (Quantitative)
• Samples: Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
• Detection Range: 11.72 pg/mL-750 pg/mL
• Detection Method: Colorimetric
• Limit of Detection: 5 pg/mL
• Usage Notes: * Do not mix components from different kit lots or use reagents beyond the kit expiration date.; * Allow all reagents to warm to room temperature for at least 30 minutes before opening.; * Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.; * Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.; * Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.; * It is recommended that all samples and standards be assayed in duplicate or triplicate.
• Preparation and Storage: Store at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

(Fig.1. Human GM-CSF Standard Curve.)

Related Product Information for GM-CSF elisa kit

Description: Human GM-CSF ELISA Kit employs a two-site sandwich ELISA to quantitate GM-CSF in samples. An antibody specific for GM-CSF has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any GM-CSF present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for GM-CSF is added to the wells. After washing, proprietary Streptavidin-HRP conjugates is added to the wells. Following a wash to remove any unbound streptavidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of GM-CSF bound in the initial step. The color development is stopped and the intensity of the color is measured.

Background: Granulocyte-macrophage colony-stimulating factor (GM-CSF) is a cytokine that stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes. GM-CSF is used therapeutically in myeloid reconstitution following bone marrow transplant, bone marrow transplant engraftment failure or delay, mobilization and following transplantation of autologous peripheral blood progenitor cells, and following induction chemotherapy in older adults with acute myelogenous leukemia (AML).

NCBI and Uniprot Product Information

• NCBI GI #: 183364
• NCBI GeneID: 1437
• UniProt Accession #: P04141
• NCBI Official Full Name: GM-CSF
• NCBI Official Synonym Full Names: colony stimulating factor 2
• NCBI Official Symbol: CSF2
• NCBI Official Synonym Symbols: CSF; GMCSF
• NCBI Protein Information: granulocyte-macrophage colony-stimulating factor
• UniProt Protein Name: Granulocyte-macrophage colony-stimulating factor
• UniProt Gene Name: CSF2
• UniProt Synonym Gene Names: GMCSF; GM-CSF; CSF
• UniProt Entry Name: CSF2_HUMAN

NCBI Description

The protein encoded by this gene is a cytokine that controls the production, differentiation, and function of granulocytes and macrophages. The active form of the protein is found extracellularly as a homodimer. This gene has been localized to a cluster of related genes at chromosome region 5q31, which is known to be associated with interstitial deletions in the 5q- syndrome and acute myelogenous leukemia. Other genes in the cluster include those encoding interleukins 4, 5, and 13. [provided by RefSeq, Jul 2008]