Rabbit CIITA Polyclonal Antibody | anti-CIITA antibody

CIITA Antibody

Cat. Num. AAA151392

• Gene Names: CIITA; C2TA; NLRA; MHC2TA; CIITAIV
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: CIITA Antibody is affinity chromatography purified via peptide column.
• Synonyms: CIITA; Polyclonal Antibody; CIITA Antibody; C2TA; NLRA; MHC2TA; CIITAIV; MHC class II transactivator; class II; major histocompatibility complex; transactivator; anti-CIITA antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: CIITA Antibody is affinity chromatography purified via peptide column.
• Form/Format: Liquid
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 1130

• Applicable Applications for anti-CIITA antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunofluorescence (IF)
• Application Notes: CIITA antibody can be used for detection of CIITA by Western blot at 1 mug/mL. Antibody can also be used for immunohistochemistry starting at 10 mug/mL. For immunofluorescence start at 20 mug/mL.; ; Optimal dilutions for each application to be determined by the researcher.; Positive Control: MBS151758 Rat Brain Tissue Lysate.
• Conjugate: Unconjugated
• Immunogen: CIITA antibody was raised against a 16 amino acid synthetic peptide near the amino terminus of human CIITA.
• Buffer: CIITA Antibody is supplied in PBS containing 0.02% sodium azide.
• Preparation and Storage: CIITA antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of CIITA in rat brain tissue lysate with CIITA antibody at 1 μg/mL.)

Immunohistochemistry (IHC)

(Immunohistochemistry of CIITA in human brain tissue with CIITA antibody at 10 μg/mL.)

Immunofluorescence (IF)

(Immunofluorescence of CIITA in human brain tissue with CIITA antibody at 20 μg/mL.)

Related Product Information for anti-CIITA antibody

CIITA Antibody: CIITA contains an acidic transcriptional activation domain, four LRRs (leucine-rich repeats) and a GTP binding domain. It is located in the nucleus and acts as a positive regulator of class II major histocompatibility complex gene transcription, and is referred to as the "master control factor" for the expression of these genes. CIITA also binds GTP and uses GTP binding to facilitate its own transport into the nucleus. Once in the nucleus it does not bind DNA but rather uses an intrinsic acetyltransferase (AT) activity to act in a coactivator-like fashion. Mutations in this gene have been associated with bare lymphocyte syndrome type II (also known as hereditary MHC class II deficiency or HLA class II-deficient combined immunodeficiency), increased susceptibility to rheumatoid arthritis, multiple sclerosis, and possibly myocardial infarction.

NCBI and Uniprot Product Information

• NCBI GI #: 218511957
• NCBI GeneID: 4261
• UniProt Accession #: P33076; Q29675; Q8SNB8; Q96KL4; A0N0N9; D3DUG0; E9PFE0
• Molecular Weight: Predicted: 124 kDa; ; Observed: 125 kDa
• NCBI Official Full Name: MHC class II transactivator
• NCBI Official Synonym Full Names: class II, major histocompatibility complex, transactivator
• NCBI Official Symbol: CIITA
• NCBI Official Synonym Symbols: C2TA; NLRA; MHC2TA; CIITAIV
• NCBI Protein Information: MHC class II transactivator; NLR family, acid domain containing; MHC class II transactivator type III; nucleotide-binding oligomerization domain, leucine rich repeat and acid domain containing
• UniProt Protein Name: MHC class II transactivator
• Protein Family: MHC class II transactivator
• UniProt Gene Name: CIITA
• UniProt Synonym Gene Names: MHC2TA; CIITA
• UniProt Entry Name: C2TA_HUMAN

NCBI Description

This gene encodes a protein with an acidic transcriptional activation domain, 4 LRRs (leucine-rich repeats) and a GTP binding domain. The protein is located in the nucleus and acts as a positive regulator of class II major histocompatibility complex gene transcription, and is referred to as the "master control factor" for the expression of these genes. The protein also binds GTP and uses GTP binding to facilitate its own transport into the nucleus. Once in the nucleus it does not bind DNA but rather uses an intrinsic acetyltransferase (AT) activity to act in a coactivator-like fashion. Mutations in this gene have been associated with bare lymphocyte syndrome type II (also known as hereditary MHC class II deficiency or HLA class II-deficient combined immunodeficiency), increased susceptibility to rheumatoid arthritis, multiple sclerosis, and possibly myocardial infarction. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2013]

Uniprot Description

CIITA: a non-DNA binding transactivator that functions both in constitutive and inducible MHC Class II expression. Defects are a cause of bare lymphocyte syndrome type II (BLS II), a form of severe combined immunodeficiency disease (SCID) characterized by a profound defect in constitutive and interferon-gamma induced MHC II expression, an absence of cellular and humoral T-cell response to antigen challenge, hypogammaglobulinemia and impaired antibody production.

Protein type: DNA-binding; Transcription, coactivator/corepressor; EC 2.7.11.1

Chromosomal Location of Human Ortholog: 16p13

Cellular Component: nucleoplasm; PML body

Molecular Function: protein C-terminus binding; protein binding; DNA binding; GTP binding; transcription activator binding; transcription coactivator activity; transferase activity, transferring acyl groups; protein complex binding; kinase activity; ATP binding

Biological Process: response to antibiotic; transcription, DNA-dependent; positive regulation of transcription, DNA-dependent; negative regulation of collagen biosynthetic process; cytokine and chemokine mediated signaling pathway; positive regulation of MHC class II biosynthetic process; positive regulation of MHC class I biosynthetic process; positive regulation of transcription from RNA polymerase II promoter; immune response; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; phosphorylation

Disease: Rheumatoid Arthritis; Bare Lymphocyte Syndrome, Type Ii