Typical Testing Data/Standard Curve (for reference only)
Human Glutamate Receptor, Ionotropic, N-Methyl-D-Aspartate 2D (GRIN2D) ELISA Kit | GRIN2D elisa kit
Human Glutamate Receptor, Ionotropic, N-Methyl-D-Aspartate 2D (GRIN2D) ELISA Kit
Gene Names
GRIN2D; EB11; NR2D; EIEE46; GluN2D; NMDAR2D
Reactivity
Human
Synonyms
Glutamate Receptor; Ionotropic; N-Methyl-D-Aspartate 2D (GRIN2D); Human Glutamate Receptor; N-Methyl-D-Aspartate 2D (GRIN2D) ELISA Kit; EXTL; Exostosin-Like 1; Glucuronosyl-N-Acetylglucosaminyl-Proteoglycan 4-Alpha-N-Acetylglucosaminyltran...; GRIN2D elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of GRIN2D. No significant cross-reactivity or interference between GRIN2D and analogues was observed.
Sequence Length
1336
Samples
Tissue homogenates and other biological fluids.
Assay Type
Sandwich
Detection Range
0.312-20ng/mL
Sensitivity
0.123ng/mL
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level GRIN2D were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level GRIN2D were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
Preparation and Storage
For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for GRIN2D elisa kit
Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of GRIN2D in human tissue homogenates and other biological fluids.
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to GRIN2D. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to GRIN2D. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain GRIN2D, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of GRIN2D in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to GRIN2D. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to GRIN2D. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain GRIN2D, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of GRIN2D in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
143,752 Da
NCBI Official Full Name
glutamate receptor ionotropic, NMDA 2D
NCBI Official Synonym Full Names
glutamate ionotropic receptor NMDA type subunit 2D
NCBI Official Symbol
GRIN2D
NCBI Official Synonym Symbols
EB11; NR2D; EIEE46; GluN2D; NMDAR2D
NCBI Protein Information
glutamate receptor ionotropic, NMDA 2D
UniProt Protein Name
Glutamate receptor ionotropic, NMDA 2D
Protein Family
UniProt Gene Name
GRIN2D
UniProt Synonym Gene Names
GluN2D; NMDAR2D; GluN2D; NMDAR2D; NR2D
NCBI Description
N-methyl-D-aspartate (NMDA) receptors are a class of ionotropic glutamate receptors. NMDA channel has been shown to be involved in long-term potentiation, an activity-dependent increase in the efficiency of synaptic transmission thought to underlie certain kinds of memory and learning. NMDA receptor channels are heteromers composed of the key receptor subunit NMDAR1 (GRIN1) and 1 or more of the 4 NMDAR2 subunits: NMDAR2A (GRIN2A), NMDAR2B (GRIN2B), NMDAR2C (GRIN2C), and NMDAR2D (GRIN2D). [provided by RefSeq, Mar 2010]
Uniprot Description
NMDA receptor subtype of glutamate-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium. Mediated by glycine.
Research Articles on GRIN2D
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Product Notes
The Human GRIN2D grin2d (Catalog #AAA453223) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA453223 ELISA Kit recognizes Human GRIN2D. It is sometimes possible for the material contained within the vial of "Glutamate Receptor, Ionotropic, N-Methyl-D-Aspartate 2D (GRIN2D), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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