Human Glutamate Receptor, Ionotropic, N-Methyl-D-Aspartate 2D (GRIN2D) RTU ELISA Kit | GRIN2D rtu elisa kit

Human Glutamate Receptor, Ionotropic, N-Methyl-D-Aspartate 2D (GRIN2D) ELISA Kit

Cat. Num. AAA459715

• Gene Names: GRIN2D; EB11; NR2D; EIEE46; GluN2D; NMDAR2D
• Reactivity: Human
• Synonyms: Glutamate Receptor; Ionotropic; N-Methyl-D-Aspartate 2D (GRIN2D); Human Glutamate Receptor; N-Methyl-D-Aspartate 2D (GRIN2D) ELISA Kit; EB11; NMDAR2D; NR2D; N-Methyl-d-Aspartate Receptor Subunit 2D; Glutamate [NMDA] receptor subunit epsilon-4; GRIN2D rtu elisa kit

• Reactivity: Human
• Sequence Length: 1336

• Assay Type: Sandwich
• Samples: Tissue Homogenates or Other Biological Fluids.
• Detection Range: 0.312-20ng/mL
• Sensitivity: 0.123ng/mL
• RTU ELISA Kit's Advantage: The Ready-To-Use (RTU) Kits require fewer steps in setup and take less time to run. Detection Solutions are pre-diluted and total incubation time is shorter than many other ELISA Kits.
• Preparation and Storage: For unopened kits: All the reagents should be kept at 4 degree C upon receipt.; For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for GRIN2D rtu elisa kit

Test Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to GRIN2D. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to GRIN2D. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain GRIN2D, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of GRIN2D in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 153946391
• NCBI GeneID: 2906
• NCBI Accession #: NP_000827.2
• NCBI GenBank Nucleotide #: NM_000836.2
• UniProt Accession #: O15399
• Molecular Weight: 143,752 Da
• NCBI Official Full Name: glutamate receptor ionotropic, NMDA 2D
• NCBI Official Synonym Full Names: glutamate ionotropic receptor NMDA type subunit 2D
• NCBI Official Symbol: GRIN2D
• NCBI Official Synonym Symbols: EB11; NR2D; EIEE46; GluN2D; NMDAR2D
• NCBI Protein Information: glutamate receptor ionotropic, NMDA 2D
• UniProt Protein Name: Glutamate receptor ionotropic, NMDA 2D
• Protein Family: Glutamate receptor ionotropic
• UniProt Gene Name: GRIN2D
• UniProt Synonym Gene Names: GluN2D; NMDAR2D; GluN2D; NMDAR2D; NR2D

NCBI Description

N-methyl-D-aspartate (NMDA) receptors are a class of ionotropic glutamate receptors. NMDA channel has been shown to be involved in long-term potentiation, an activity-dependent increase in the efficiency of synaptic transmission thought to underlie certain kinds of memory and learning. NMDA receptor channels are heteromers composed of the key receptor subunit NMDAR1 (GRIN1) and 1 or more of the 4 NMDAR2 subunits: NMDAR2A (GRIN2A), NMDAR2B (GRIN2B), NMDAR2C (GRIN2C), and NMDAR2D (GRIN2D). [provided by RefSeq, Mar 2010]

Uniprot Description

Component of NMDA receptor complexes that function as heterotetrameric, ligand-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium. Channel activation requires binding of the neurotransmitter glutamate to the epsilon subunit, glycine binding to the zeta subunit, plus membrane depolarization to eliminate channel inhibition by Mg2+ (PubMed:9489750, PubMed:27616483, PubMed:26875626, PubMed:28126851). Sensitivity to glutamate and channel kinetics depend on the subunit composition (PubMed:9489750).