Human Glutamate Receptor, Ionotropic, AMPA 2 (GRIA2) ELISA Kit | GRIA2 elisa kit

Human Glutamate Receptor, Ionotropic, AMPA 2 (GRIA2) ELISA Kit

Cat. Num. AAA2705625

• Gene Names: GRIA2; GLUR2; GLURB; GluA2; HBGR2; GluR-K2
• Reactivity: Human
• Synonyms: Glutamate Receptor; Ionotropic; AMPA 2 (GRIA2); Human Glutamate Receptor; AMPA 2 (GRIA2) ELISA Kit; GLUR2; GLURB; HBGR2; GluR-K2; AMPA-selective glutamate receptor 2; GRIA2 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Glutamate Receptor, Ionotropic, AMPA 2 (GRIA2).; No significant cross-reactivity or interference between Glutamate Receptor, Ionotropic, AMPA 2 (GRIA2) and analogues was observed.
• Sequence Length: 836

• Assay Type: Double-antibody Sandwich
• Samples: Tissue Homogenates, Cell Lysates, Cell Culture Supernates and Other Biological Fluids
• Detection Range: 0.312-20ng/mL
• Sensitivity: < 0.116ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Glutamate Receptor, Ionotropic, AMPA 2 (GRIA2) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Glutamate Receptor, Ionotropic, AMPA 2 (GRIA2) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for GRIA2 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glutamate Receptor, Ionotropic, AMPA 2 (GRIA2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Glutamate Receptor, Ionotropic, AMPA 2 (GRIA2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Glutamate Receptor, Ionotropic, AMPA 2 (GRIA2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Glutamate Receptor, Ionotropic, AMPA 2 (GRIA2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 119625275
• NCBI GeneID: 2891
• UniProt Accession #: P42262; Q96FP6; A8MT92; I6L997
• Molecular Weight: 93,775 Da
• NCBI Official Full Name: glutamate receptor, ionotropic, AMPA 2, isoform CRA_f
• NCBI Official Synonym Full Names: glutamate ionotropic receptor AMPA type subunit 2
• NCBI Official Symbol: GRIA2
• NCBI Official Synonym Symbols: GLUR2; GLURB; GluA2; HBGR2; GluR-K2
• NCBI Protein Information: glutamate receptor 2
• UniProt Protein Name: Glutamate receptor 2
• UniProt Gene Name: GRIA2
• UniProt Synonym Gene Names: GLUR2; GluR-2; GluA2

NCBI Description

Glutamate receptors are the predominant excitatory neurotransmitter receptors in the mammalian brain and are activated in a variety of normal neurophysiologic processes. This gene product belongs to a family of glutamate receptors that are sensitive to alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate (AMPA), and function as ligand-activated cation channels. These channels are assembled from 4 related subunits, GRIA1-4. The subunit encoded by this gene (GRIA2) is subject to RNA editing (CAG->CGG; Q->R) within the second transmembrane domain, which is thought to render the channel impermeable to Ca(2+). Human and animal studies suggest that pre-mRNA editing is essential for brain function, and defective GRIA2 RNA editing at the Q/R site may be relevant to amyotrophic lateral sclerosis (ALS) etiology. Alternative splicing, resulting in transcript variants encoding different isoforms, (including the flip and flop isoforms that vary in their signal transduction properties), has been noted for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

Receptor for glutamate that functions as ligand-gated ion channel in the central nervous system and plays an important role in excitatory synaptic transmission. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L-glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist. In the presence of CACNG4 or CACNG7 or CACNG8, shows resensitization which is characterized by a delayed accumulation of current flux upon continued application of glutamate. Through complex formation with NSG1, GRIP1 and STX12 controls the intracellular fate of AMPAR and the endosomal sorting of the GRIA2 subunit toward recycling and membrane targeting ().