Human Fibronectin Leucine Rich Transmembrane Protein 3 (FLRT3) ELISA Kit | FLRT3 elisa kit

Human Fibronectin Leucine Rich Transmembrane Protein 3 (FLRT3) ELISA Kit

Cat. Num. AAA2705814

• Gene Names: FLRT3; HH21
• Reactivity: Human
• Synonyms: Fibronectin Leucine Rich Transmembrane Protein 3 (FLRT3); Human Fibronectin Leucine Rich Transmembrane Protein 3 (FLRT3) ELISA Kit; Fibronectin-like domain-containing leucine-rich transmembrane protein 3; Leucine-rich repeat transmembrane protein FLRT3; FLRT3 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Fibronectin Leucine Rich Transmembrane Protein 3 (FLRT3).; No significant cross-reactivity or interference between Fibronectin Leucine Rich Transmembrane Protein 3 (FLRT3) and analogues was observed.
• Sequence Length: 649

• Assay Type: Double-antibody Sandwich
• Samples: Tissue Homogenates, Cell Lysates and Other Biological Fluids
• Detection Range: 1.56-100ng/mL
• Sensitivity: < 0.57ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Fibronectin Leucine Rich Transmembrane Protein 3 (FLRT3) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Fibronectin Leucine Rich Transmembrane Protein 3 (FLRT3) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for FLRT3 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Fibronectin Leucine Rich Transmembrane Protein 3 (FLRT3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Fibronectin Leucine Rich Transmembrane Protein 3 (FLRT3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Fibronectin Leucine Rich Transmembrane Protein 3 (FLRT3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Fibronectin Leucine Rich Transmembrane Protein 3 (FLRT3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 18088788
• NCBI GeneID: 23767
• UniProt Accession #: Q9NZU0; Q542Z9; Q96K39; Q96K42; Q96KB1; Q9P259; D3DW20
• Molecular Weight: 73,004 Da
• NCBI Official Full Name: Fibronectin leucine rich transmembrane protein 3
• NCBI Official Synonym Full Names: fibronectin leucine rich transmembrane protein 3
• NCBI Official Symbol: FLRT3
• NCBI Official Synonym Symbols: HH21
• NCBI Protein Information: leucine-rich repeat transmembrane protein FLRT3
• UniProt Protein Name: Leucine-rich repeat transmembrane protein FLRT3
• Protein Family: Leucine-rich repeat transmembrane protein
• UniProt Gene Name: FLRT3
• UniProt Synonym Gene Names: KIAA1469

NCBI Description

This gene encodes a member of the fibronectin leucine rich transmembrane protein (FLRT) family. FLRTs may function in cell adhesion and/or receptor signalling. Their protein structures resemble small leucine-rich proteoglycans found in the extracellular matrix. This gene is expressed in many tissues. Two alternatively spliced transcript variants encoding the same protein have been described for this gene. [provided by RefSeq, Jul 2010]

Uniprot Description

Functions in cell-cell adhesion, cell migration and axon guidance, exerting an attractive or repulsive role depending on its interaction partners. Plays a role in the spatial organization of brain neurons. Plays a role in vascular development in the retina (). Plays a role in cell-cell adhesion via its interaction with ADGRL3 and probably also other latrophilins that are expressed at the surface of adjacent cells (PubMed:26235030). Interaction with the intracellular domain of ROBO1 mediates axon attraction towards cells expressing NTN1. Mediates axon growth cone collapse and plays a repulsive role in neuron guidance via its interaction with UNC5B, and possibly also other UNC-5 family members (). Promotes neurite outgrowth (in vitro) (PubMed:14706654). Mediates cell-cell contacts that promote an increase both in neurite number and in neurite length. Plays a role in the regulation of the density of glutamaergic synapses. Plays a role in fibroblast growth factor-mediated signaling cascades. Required for normal morphogenesis during embryonic development, but not for normal embryonic patterning. Required for normal ventral closure, headfold fusion and definitive endoderm migration during embryonic development. Required for the formation of a normal basement membrane and the maintenance of a normal anterior visceral endoderm during embryonic development ().