Canine Adenylate Cyclase 3 ELISA Kit | ADCY3 elisa kit

Canine Adenylate Cyclase 3 ELISA Kit

Cat. Num. AAA745705

• Gene Names: ADCY3; AC3
• Reactivity: Canine
• Synonyms: Adenylate Cyclase 3; Canine Adenylate Cyclase 3 ELISA Kit; ADCY3 elisa kit

• Reactivity: Canine

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Detection Range: 1.0-25ng/mL
• Sensitivity: 0.1ng/mL
• Intended Uses: This ADCY3 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Canine ADCY3. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ADCY3 elisa kit

Principle of the assay: ADCY3 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ADCY3 antibody and an ADCY3-HRP conjugate. The assay sample and buffer are incubated together with ADCY3-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ADCY3 concentration since ADCY3 from samples and ADCY3-HRP conjugate compete for the anti-ADCY3 antibody binding site. Since the number of sites is limited, as more sites are occupied by ADCY3 from the sample, fewer sites are left to bind ADCY3-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ADCY3 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for ADCY3 elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 219519026
• NCBI GeneID: 109
• Molecular Weight: 82,289 Da
• NCBI Official Full Name: ADCY3 protein
• NCBI Official Synonym Full Names: adenylate cyclase 3
• NCBI Official Symbol: ADCY3
• NCBI Official Synonym Symbols: AC3
• NCBI Protein Information: adenylate cyclase type 3; AC-III; adenylyl cyclase 3; ATP pyrophosphate-lyase 3; adenylate cyclase type III; adenylyl cyclase, type III; adenylate cyclase, olfactive type
• UniProt Protein Name: Adenylate cyclase type 3
• Protein Family: Adenylate cyclase
• UniProt Gene Name: ADCY3
• UniProt Synonym Gene Names: KIAA0511; AC-III; AC3
• UniProt Entry Name: ADCY3_HUMAN

NCBI Description

This gene encodes adenylyl cyclase 3 which is a membrane-associated enzyme and catalyzes the formation of the secondary messenger cyclic adenosine monophosphate (cAMP). This protein appears to be widely expressed in various human tissues and may be involved in a number of physiological and pathophysiological metabolic processes. [provided by RefSeq, Jul 2008]

Uniprot Description

ADCY3: a membrane-associated enzyme that catalyzes the formation of the secondary messenger cyclic adenosine monophosphate (cAMP). Widely expressed in various human tissues and may be involved in a number of physiological and pathophysiological metabolic processes. Involved in odorant detection (possibly) via modulation of intracellular cAMP concentration.

Protein type: Transporter; Nucleotide Metabolism - purine; Membrane protein, multi-pass; Adenylyl cyclase; EC 4.6.1.1; Lyase; Membrane protein, integral; Transporter, aquaporin family

Chromosomal Location of Human Ortholog: 2p23.3

Cellular Component: membrane; integral to plasma membrane; cytoplasm; plasma membrane; cilium

Molecular Function: calmodulin binding; metal ion binding; ATP binding; adenylate cyclase activity

Biological Process: epidermal growth factor receptor signaling pathway; fibroblast growth factor receptor signaling pathway; nerve growth factor receptor signaling pathway; water transport; sensory perception of smell; activation of protein kinase A; signal transduction; G-protein signaling, adenylate cyclase activating pathway; synaptic transmission; phospholipase C activation; energy reserve metabolic process; innate immune response; renal water homeostasis; cAMP biosynthetic process; G-protein signaling, adenylate cyclase inhibiting pathway; transmembrane transport