Chicken Small nuclear ribonucleoprotein ELISA Kit | SnRNP elisa kit

Chicken Small nuclear ribonucleoprotein ELISA Kit

Cat. Num. AAA746910

• Gene Names: LSM2; G7B; snRNP; C6orf28; YBL026W
• Reactivity: Chicken
• Synonyms: Small nuclear ribonucleoprotein; Chicken Small nuclear ribonucleoprotein ELISA Kit; SnRNP elisa kit

• Reactivity: Chicken

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Intended Uses: This SnRNP ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Chicken SnRNP. This ELISA kit for research use only, not for therapeutic applications
• !Intended Uses: This SnRNP ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Chicken SnRNP. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for SnRNP elisa kit

Principle of the Assay: SnRNP ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-SnRNP antibody and an SnRNP-HRP conjugate. The assay sample and buffer are incubated together with SnRNP-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the SnRNP concentration since SnRNP from samples and SnRNP-HRP conjugate compete for the anti-SnRNP antibody binding site. Since the number of sites is limited, as more sites are occupied by SnRNP from the sample, fewer sites are left to bind SnRNP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SnRNP concentration in each sample is interpolated from this standard curve.

Product Categories/Family for SnRNP elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 4529895
• NCBI GeneID: 57819
• Molecular Weight: 10,835 Da
• NCBI Official Full Name: snRNP
• NCBI Official Synonym Full Names: LSM2 homolog, U6 small nuclear RNA associated (S. cerevisiae)
• NCBI Official Symbol: LSM2
• NCBI Official Synonym Symbols: G7B; snRNP; C6orf28; YBL026W
• NCBI Protein Information: U6 snRNA-associated Sm-like protein LSm2; protein G7b; snRNP core Sm-like protein Sm-x5; small nuclear ribonuclear protein D homolog
• UniProt Protein Name: U6 snRNA-associated Sm-like protein LSm2
• Protein Family: Small nuclear ribonucleoprotein
• UniProt Gene Name: LSM2
• UniProt Synonym Gene Names: C6orf28; G7B
• UniProt Entry Name: LSM2_HUMAN

NCBI Description

This gene encodes a member of the LSm family of RNA-binding proteins. LSm proteins form stable heteromers that bind specifically to the 3'-terminal oligo(U) tract of U6 snRNA and may play a role in pre-mRNA splicing by mediating U4/U6 snRNP formation. Pseudogenes of this gene are located on the short arm of chromosomes 6 and 19. [provided by RefSeq, Nov 2011]

Uniprot Description

LSM2: Binds specifically to the 3'-terminal U-tract of U6 snRNA. May be involved in pre-mRNA splicing. Identified in the spliceosome C complex. LSm subunits form a heteromer with a doughnut shape. Belongs to the snRNP Sm proteins family.

Protein type: RNA-binding; RNA splicing; Spliceosome

Chromosomal Location of Human Ortholog: 6p21.3

Cellular Component: nucleoplasm; cytosol

Molecular Function: protein binding; Ral GTPase binding; protein kinase binding; U6 snRNA binding

Biological Process: nuclear mRNA splicing, via spliceosome; RNA splicing; gene expression; mRNA catabolic process, deadenylation-dependent decay