Typical Testing Data/Standard Curve
Rat Fibroblast Growth Factor 19 ELISA Kit | FGF19 elisa kit
Rat Fibroblast Growth Factor 19 ELISA Kit
Typical Testing Data/Standard Curve
Typical Testing Data/Standard Curve
Principle of the Assay||FGF-19 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-FGF-19 antibody and an FGF-19-HRP conjugate. The assay sample and buffer are incubated together with FGF-19-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the FGF-19 concentration since FGF-19 from samples and FGF-19-HRP conjugate compete for the anti-FGF-19 antibody binding site. Since the number of sites is limited, as more sites are occupied by FGF-19 from the sample, fewer sites are left to bind FGF-19-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The FGF-19 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes including embryonic development cell growth, morphogenesis, tissue repair, tumor growth and invasion. This growth factor is a high affinity, heparin dependent ligand for FGFR4. Expression of this gene was detected only in fetal but not adult brain tissue. Synergistic interaction of the chick homolog and Wnt-8c has been shown to be required for initiation of inner ear development. [provided by RefSeq, Jul 2008]
Uniprot Description
FGF19: Involved in the suppression of bile acid biosynthesis through down-regulation of CYP7A1 expression, following positive regulation of the JNK and ERK1/2 cascades. Stimulates glucose uptake in adipocytes. Activity requires the presence of KLB and FGFR4. Belongs to the heparin-binding growth factors family.
Protein type: Cytokine; Motility/polarity/chemotaxis; Cell development/differentiation; Secreted, signal peptide; Secreted
Chromosomal Location of Human Ortholog: 11q13.1
Cellular Component: extracellular region
Molecular Function: protein binding; growth factor activity; fibroblast growth factor receptor binding
Biological Process: epidermal growth factor receptor signaling pathway; nervous system development; fibroblast growth factor receptor signaling pathway; phosphoinositide-mediated signaling; nerve growth factor receptor signaling pathway; heart development; positive regulation of JNK cascade; positive regulation of glucose import; positive regulation of cell proliferation; insulin receptor signaling pathway; innate immune response; positive regulation of protein amino acid phosphorylation; neural crest cell migration
Research Articles on FGF19
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Product Notes
The Rat FGF19 fgf19 (Catalog #AAA746153) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA746153 ELISA Kit recognizes Rat FGF19. It is sometimes possible for the material contained within the vial of "Fibroblast Growth Factor 19, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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