Human Fibroblast Growth Factor 12 (FGF12) ELISA Kit | FGF12 elisa kit

Human Fibroblast Growth Factor 12 (FGF12) ELISA Kit

Cat. Num. AAA450406

• Gene Names: FGF12; FHF1; EIEE47; FGF12B
• Reactivity: Human
• Synonyms: Fibroblast Growth Factor 12 (FGF12); Human Fibroblast Growth Factor 12 (FGF12) ELISA Kit; FGF12B; FHF1; Fibroblast Growth Factor Homologous Factor 1; Myocyte-Activating Factor; FGF12 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of FGF10. No significant cross-reactivity or interference between FGF10 and analogues was observed.
• Sequence Length: 181

• Samples: Mouse Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates Or Other Biological Fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 15.6-1000pg/mL
• Sensitivity: 5.3pg/mL.
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level FGF10 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level FGF10 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for FGF12 elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of FGF10 in mouse serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to FGF10. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to FGF10. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain FGF10, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of FGF10 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 21614511
• NCBI GeneID: 2257
• NCBI Accession #: NP_004104.3
• NCBI GenBank Nucleotide #: NM_004113.5
• UniProt Accession #: P61328; O35339; P70376; Q8TBG5; Q92912; Q93001; B2R6B7; B2R976
• Molecular Weight: 20,424 Da
• NCBI Official Full Name: fibroblast growth factor 12 isoform 2
• NCBI Official Synonym Full Names: fibroblast growth factor 12
• NCBI Official Symbol: FGF12
• NCBI Official Synonym Symbols: FHF1; EIEE47; FGF12B
• NCBI Protein Information: fibroblast growth factor 12
• UniProt Protein Name: Fibroblast growth factor 12
• Protein Family: Fibroblast growth factor
• UniProt Gene Name: FGF12
• UniProt Synonym Gene Names: FGF12B; FHF1; FGF-12; FHF-1

NCBI Description

The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth, and invasion. This growth factor lacks the N-terminal signal sequence present in most of the FGF family members, but it contains clusters of basic residues that have been demonstrated to act as a nuclear localization signal. When transfected into mammalian cells, this protein accumulated in the nucleus, but was not secreted. The specific function of this gene has not yet been determined. Two alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008]

Uniprot Description

Involved in nervous system development and function. Involved in the positive regulation of voltage-gated sodium channel activity. Promotes neuronal excitability by elevating the voltage dependence of neuronal sodium channel SCN8A fast inactivation.