Chicken Axin-1 ELISA Kit | AXIN1 elisa kit

Chicken Axin-1 ELISA Kit

Cat. Num. AAA288290

• Gene Names: AXIN1; AXIN; AXIN1-RGS
• Reactivity: Chicken
• Synonyms: Axin-1; Chicken Axin-1 ELISA Kit; AXIN1 elisa kit

• Reactivity: Chicken
• Specificity: This assay recognizes recombinant and natural Chicken Axin-1. No significant cross-reactivity or interference was observed.
• Sequence Length: 826

• Samples: Serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.
• Assay Type: Competitive
• Detection Range: 0.31-20.0 ng/ml.
• Preparation and Storage: For long term storage, please store the entire kit at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for AXIN1 elisa kit

Principle of the Assay: The ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Axin-1, During the reaction, Axin-1 in the sample or standard competes with a fixed amount of biotin-labeled Axin-1 for sites on a pre-coated Monoclonal antibody specific to Axin-1. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of Axin-1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 45384430
• NCBI GeneID: 395786
• NCBI Accession #: NP_990275.1
• NCBI GenBank Nucleotide #: NM_204944.2
• UniProt Accession #: O42400
• Molecular Weight: 94,932 Da
• NCBI Official Full Name: axin-1
• NCBI Official Synonym Full Names: axin 1
• NCBI Official Symbol: AXIN1
• NCBI Official Synonym Symbols: AXIN; AXIN1-RGS
• NCBI Protein Information: axin-1
• UniProt Protein Name: Axin-1
• Protein Family: Axin
• UniProt Gene Name: AXIN1
• UniProt Synonym Gene Names: AXIN; AXN
• UniProt Entry Name: AXIN1_CHICK

Uniprot Description

Controls dorsoventral patterning via two opposing effects; down-regulates beta-catenin to inhibit the Wnt signaling pathway and ventralize embryos, but also dorsalizes embryos by activating a Wnt-independent JNK signaling pathway.