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Typical Testing Data/Standard Curve (for reference only)

Rat Fibroblast Growth Factor (FGF) ELISA Kit | FGF elisa kit

Rat Fibroblast Growth Factor (FGF) ELISA KIT

Gene Names
FGF12; FHF1; FGF12B
Reactivity
Rat
Synonyms
Fibroblast Growth Factor (FGF); Rat Fibroblast Growth Factor (FGF) ELISA KIT; FGF elisa kit
Ordering
For Research Use Only!
Reactivity
Rat
Sequence Length
174
Samples
Rat serum, plasma or cell culture supernatant
Assay Type
Quantitative Sandwich
Detection Range
1000 pg/mL-15.6 pg/mL
Sensitivity
Up to 5 pg/mL
Intra-assay Precision
<= 8%
Inter-assay Precision
<= 12%
Preparation and Storage
Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Typical Testing Data/Standard Curve (for reference only)
Related Product Information for FGF elisa kit
Principle of the Assay: As mentioned above, this kit utilizes the Double Antibody Sandwich ELISA technique. The pre-coated antibody is an anti-Rat FGF monoclonal antibody, while the detection antibody is a biotinylated polyclonal antibody. Samples and biotinylated antibodies are added into ELISA plate wells and washed out with PBS or TBS after their respective additions to the wells. Then Avidin-peroxidase conjugates are added to the wells in after. TMB substrate is used for coloration after the enzyme conjugate has already been thoroughly washed out of the wells by PBS or TBS. TMB reacts to form a blue product from the peroxidase activity, and finally turns to yellow after addition of the stop solution (Color Reagent C). The color intensity and quantity of target analyte in the sample are positively correlated.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
Molecular Weight
20,424 Da
NCBI Official Full Name
fibroblast growth factor
NCBI Official Synonym Full Names
fibroblast growth factor 12
NCBI Official Symbol
FGF12
NCBI Official Synonym Symbols
FHF1; FGF12B
NCBI Protein Information
fibroblast growth factor 12; FHF-1; FGF-12; myocyte-activating factor; fibroblast growth factor 12B; fibroblast growth factor FGF-12b; fibroblast growth factor homologous factor 1
UniProt Protein Name
Fibroblast growth factor 12
Protein Family
UniProt Gene Name
FGF12
UniProt Synonym Gene Names
FGF12B; FHF1; FGF-12; FHF-1
UniProt Entry Name
FGF12_HUMAN

NCBI Description

The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth, and invasion. This growth factor lacks the N-terminal signal sequence present in most of the FGF family members, but it contains clusters of basic residues that have been demonstrated to act as a nuclear localization signal. When transfected into mammalian cells, this protein accumulated in the nucleus, but was not secreted. The specific function of this gene has not yet been determined. Two alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008]

Uniprot Description

FGF12: Probably involved in nervous system development and function. Belongs to the heparin-binding growth factors family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cytokine; Cell development/differentiation

Chromosomal Location of Human Ortholog: 3q28

Cellular Component: extracellular space; nucleus

Molecular Function: heparin binding; sodium channel regulator activity; growth factor activity; fibroblast growth factor receptor binding

Biological Process: synaptic transmission; nervous system development; fibroblast growth factor receptor signaling pathway; cell-cell signaling; adult locomotory behavior; neuromuscular process; heart development; JNK cascade; signal transduction

Research Articles on FGF

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Product Notes

The Rat FGF fgf12 (Catalog #AAA260671) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA260671 ELISA Kit recognizes Rat FGF. It is sometimes possible for the material contained within the vial of "Fibroblast Growth Factor (FGF), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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