Human FGF2 ELISA Kit | FGF2 elisa kit

Human FGF2 PicoKine ELISA Kit

Cat. Num. AAA1751537

• Gene Names: FGF2; BFGF; FGFB; FGF-2; HBGF-2
• Reactivity: Human
• Synonyms: FGF2; Human FGF2 PicoKine ELISA Kit; Human FGF2; FGF2 elisa kit

• Reactivity: Human
• Specificity: Natural and recombinant human FGF2
• Sequence Length: 155

• Samples: Cell Culture Supernates, Serum, Plasma(Heparin, Edta) And Cell Lysates
• Intra-assay Precision: Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested on one plate to assess intra-assay precision
• Inter-assay Precision: Inter-Assay Precision (Precision accross assays):Three samples of known concentration were tested in separate assays to assess inter-assay precision.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for FGF2 elisa kit

Principle of the Assay: The Picokine Human FABP4 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Human FABP4 with a 96-well strip plate that is pre-coated with antibody specific for FABP4. The detection antibody is a biotinylated antibody specific for FABP4. The kit contains recombinant Human FABP4 with immunogen: Expression system for standard: E.coli; Immunogen sequence: M1-A132. The kit is analytically validated with ready to use reagents. To measure Human FABP4, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly propotional to Human FABP4 in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Human FABP4 in the sample!!Background/Introduction: FABP4, also known as aP2 (adipocyte Protein 2), encodes the fatty acid binding protein found in adipocytes. The FABP4 gene is mapped to 8q21. FABP4 functions as a cytosolic lipid chaperone in macrophages and is involved in regulating macrophage endoplasmic reticulum (ER) stress. Aditionally, FABP4 protein is expressed in HBEs and is strongly upregulated by both IL4 and IL13 and downregulated by IFN-gamma, suggesting a role in allergic airway inflammation.

NCBI and Uniprot Product Information

• NCBI GI #: 1371565425
• NCBI GeneID: 2247
• NCBI Accession #: NP_001348594.1
• NCBI GenBank Nucleotide #: NM_001361665.1
• NCBI Official Full Name: fibroblast growth factor 2 isoform 18 kDa
• NCBI Official Synonym Full Names: fibroblast growth factor 2
• NCBI Official Symbol: FGF2
• NCBI Official Synonym Symbols: BFGF; FGFB; FGF-2; HBGF-2
• NCBI Protein Information: fibroblast growth factor 2
• Protein Family: Fibroblast growth factor

NCBI Description

The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members bind heparin and possess broad mitogenic and angiogenic activities. This protein has been implicated in diverse biological processes, such as limb and nervous system development, wound healing, and tumor growth. The mRNA for this gene contains multiple polyadenylation sites, and is alternatively translated from non-AUG (CUG) and AUG initiation codons, resulting in five different isoforms with distinct properties. The CUG-initiated isoforms are localized in the nucleus and are responsible for the intracrine effect, whereas, the AUG-initiated form is mostly cytosolic and is responsible for the paracrine and autocrine effects of this FGF. [provided by RefSeq, Jul 2008]