Typical Testing Data/Standard Curve (for reference only)
Human Fetuin A ELISA Kit
Fetuin A ELISA
Reactivity
Human
Synonyms
Fetuin A; Fetuin A ELISA; Fetuin A elisa kit
Reactivity
Human
Samples
Rat Serum, Body Fluids, Tissue Lysates Or Cell Culture Supernates
Assay Type
Sandwich
Detection Range
0 uIU/ml-140 uIU/ml
Sensitivity
< 5 uIU/ml
Preparation and Storage
2 - 8 degree C
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for Fetuin A elisa kit
Intended Uses: For determination of Insulin in rat serum, body fluids, tissue lysates or cell culture supernates. For research use only.
Principle of the Assay: This kit is based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Insulin monoclonal antibody is pre-coated onto 96-well plates. HRP conjugated anti-Insulin polyclonal antibody are used as detection antibodies. The standards, test samples and HRP conjugated detection antibody are added to the wells subsequently, and wash with wash buffer. TMB substrates are used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow is proportional to the Insulin amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Insulin can be calculated!!Background/Introduction: Insulin, synthesized by the beta cells of the islets of Langerhans, consists of 2 dissimilar polypeptide chains, A and B, which are linked by 2 disulfide bonds. The human insulin gene contains 3 exons; exon 2 encodes the signal peptide, the B chain, and part of the C peptide, while exon 3 encodes the remainder of the C peptide and the A chain. Insulin has a potent acute antiinflammatory effect, including a reduction in intranuclear NF-kappa-B, an increase in IKB, and decreases in the generation of reactive oxygen species. It causes cells in the liver, muscle, and fat tissue to take up glucose from the blood, storing it as glycogen inside these tissues, and improved insulin-stimulated glucose uptake after endurance training results from hemodynamic adaptations as well as increased cellular protein content of individual insulin signaling components and molecules involved in glucose transport and metabolism.
Principle of the Assay: This kit is based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Insulin monoclonal antibody is pre-coated onto 96-well plates. HRP conjugated anti-Insulin polyclonal antibody are used as detection antibodies. The standards, test samples and HRP conjugated detection antibody are added to the wells subsequently, and wash with wash buffer. TMB substrates are used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow is proportional to the Insulin amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Insulin can be calculated!!Background/Introduction: Insulin, synthesized by the beta cells of the islets of Langerhans, consists of 2 dissimilar polypeptide chains, A and B, which are linked by 2 disulfide bonds. The human insulin gene contains 3 exons; exon 2 encodes the signal peptide, the B chain, and part of the C peptide, while exon 3 encodes the remainder of the C peptide and the A chain. Insulin has a potent acute antiinflammatory effect, including a reduction in intranuclear NF-kappa-B, an increase in IKB, and decreases in the generation of reactive oxygen species. It causes cells in the liver, muscle, and fat tissue to take up glucose from the blood, storing it as glycogen inside these tissues, and improved insulin-stimulated glucose uptake after endurance training results from hemodynamic adaptations as well as increased cellular protein content of individual insulin signaling components and molecules involved in glucose transport and metabolism.
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Product Notes
The Human Fetuin A (Catalog #AAA494773) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA494773 ELISA Kit recognizes Human Fetuin A. It is sometimes possible for the material contained within the vial of "Fetuin A, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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