Human Factor XI Antigen ELISA Kit
Factor XI Antigen ELISA Kit
Reactivity
Human
Synonyms
Factor XI Antigen; Factor XI Antigen ELISA Kit; Factor XI Antigen elisa kit
Reactivity
Human
Related Product Information for Factor XI Antigen elisa kit
The VisuLize Factor XI ELISA Kit is an Enzyme Immunoassay for the quantitative determination of Factor XI Antigen in human plasma samples using the double antibody enzyme linked immuno-sorbent assay (ELISA). This Factor XI ELISA Kit is for Research Use Only and must not be used for test applications.
Factor XI (F11, FXI) is a coagulation protein produced in the liver that circulates in plasma at approximately 5 ug/ml (30 nM). The mass of FXI is 160 kDa as determined by SDS-PAGE under nonreducing conditions and 80 kDa upon reduction. FXI consists of two identical 80 kDa subunits linked by disulphide bonds. Each subunit consists of a tandem repeat of four apple domains followed by a serine protease catalytic domain. Cleavage of FXI by activated factor XII or thrombin converts each subunit into a two-chain form and generates two active sites per FXIa molecule. The mass of FXIa is 160 kDa unreduced, but upon reduction FXIa migrates as a heavy chain of 50 kDa and a light chain of 30 kDa. The catalytic site of FXIa resides in the light chain. In plasma, FXI or FXIa circulates in non-covalent 1:1 complex with high molecular weight kininogen, which acts as a cofactor in the activation of FXI by activated factor XII. The activity of FXIa is regulated by platelets and by several proteinase inhibitors including, in order of decreasing importance, C1-inhibitor, alpha-2-antiplasmin, alpha-1-antitrypsin and antithrombin. Heparin has relatively little effect on the rate of inhibition of FXIa by antithrombin. The only known natural substrate for activated FXI (FXIa) is factor lX (Christmas factor) and the only cofactor required for this reaction is ionized calcium1-3.
Factor XI (F11, FXI) is a coagulation protein produced in the liver that circulates in plasma at approximately 5 ug/ml (30 nM). The mass of FXI is 160 kDa as determined by SDS-PAGE under nonreducing conditions and 80 kDa upon reduction. FXI consists of two identical 80 kDa subunits linked by disulphide bonds. Each subunit consists of a tandem repeat of four apple domains followed by a serine protease catalytic domain. Cleavage of FXI by activated factor XII or thrombin converts each subunit into a two-chain form and generates two active sites per FXIa molecule. The mass of FXIa is 160 kDa unreduced, but upon reduction FXIa migrates as a heavy chain of 50 kDa and a light chain of 30 kDa. The catalytic site of FXIa resides in the light chain. In plasma, FXI or FXIa circulates in non-covalent 1:1 complex with high molecular weight kininogen, which acts as a cofactor in the activation of FXI by activated factor XII. The activity of FXIa is regulated by platelets and by several proteinase inhibitors including, in order of decreasing importance, C1-inhibitor, alpha-2-antiplasmin, alpha-1-antitrypsin and antithrombin. Heparin has relatively little effect on the rate of inhibition of FXIa by antithrombin. The only known natural substrate for activated FXI (FXIa) is factor lX (Christmas factor) and the only cofactor required for this reaction is ionized calcium1-3.
Product Categories/Family for Factor XI Antigen elisa kit
References
1. Wuillemin WA, Minnema M, Meijers JCM, Roem D, Erenberg AJM, Nuijens JH, ten Cate H, Hack EC; Inactivation of Factor XIa in Human Plasma Assessed by Measuring Factor XIa-Protease lnhibitor Complexes: Major Role for Cl-lnhibitor. Blood 85:1517, 1995.
2. DeLa Cadena R, Watchtfogel YT, Colman RW, in Hemostasis and Thrombosis, 3d Edition, eds. RW Colman, J Hirsh, VJ Marder and EW Salzman, pp. 219-240, J.B. Lippincott Co., Philadelphia, 1994.
3. Baglia FA, Seaman FS, Walsh, PN; The Apple l and 4 domains of Factor XI Act to Synergistically Promote the Surface-Mediated Activation of Factor XI by Factor XIIa. Blood 85:2078, 1995.
2. DeLa Cadena R, Watchtfogel YT, Colman RW, in Hemostasis and Thrombosis, 3d Edition, eds. RW Colman, J Hirsh, VJ Marder and EW Salzman, pp. 219-240, J.B. Lippincott Co., Philadelphia, 1994.
3. Baglia FA, Seaman FS, Walsh, PN; The Apple l and 4 domains of Factor XI Act to Synergistically Promote the Surface-Mediated Activation of Factor XI by Factor XIIa. Blood 85:2078, 1995.
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Product Notes
The Human Factor XI Antigen (Catalog #AAA514070) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA514070 ELISA Kit recognizes Human Factor XI Antigen. It is sometimes possible for the material contained within the vial of "Factor XI Antigen, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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