Typical Testing Data/Standard Curve (for reference only)
Human Eukaryotic Translation Initiation Factor 6 (EIF6) ELISA Kit | EIF6 elisa kit
Human Eukaryotic Translation Initiation Factor 6 (EIF6) ELISA Kit
Gene Names
Eif6; CAB; eIF-6; p27BBP; Itgb4bp; imc-415; AA408895; p27(BBP); 1110004P11
Reactivity
Human
Synonyms
Eukaryotic Translation Initiation Factor 6 (EIF6); Human Eukaryotic Translation Initiation Factor 6 (EIF6) ELISA Kit; ITGB4BP; CAB; p27BBP; Integrin Beta 4 Binding Protein; B4 integrin interactor; EIF6 elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of EIF3G. No significant cross-reactivity or interference between EIF3G and analogues was observed.
Sequence Length
245
Samples
Human Tissue Homogenates, Cell Lysates And Other Biological Fluids
Assay Type
Quantitative Sandwich
Detection Range
0.312-20ng/mL
Sensitivity
0.117ng/mL.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level EIF3G were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level EIF3G were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for EIF6 elisa kit
Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of EIF3G in human tissue homogenates, cell lysates and other biological fluids.
Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to EIF3G. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to EIF3G. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain EIF3G, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of EIF3G in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to EIF3G. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to EIF3G. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain EIF3G, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of EIF3G in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Product Categories/Family for EIF6 elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
26,511 Da
NCBI Official Full Name
eukaryotic translation initiation factor 6
NCBI Official Synonym Full Names
eukaryotic translation initiation factor 6
NCBI Official Symbol
Eif6
NCBI Official Synonym Symbols
CAB; eIF-6; p27BBP; Itgb4bp; imc-415; AA408895; p27(BBP); 1110004P11
NCBI Protein Information
eukaryotic translation initiation factor 6
UniProt Protein Name
Eukaryotic translation initiation factor 6
Protein Family
UniProt Gene Name
Eif6
UniProt Synonym Gene Names
Itgb4bp; eIF-6
UniProt Entry Name
IF6_MOUSE
NCBI Description
The protein encoded by this gene is a eukaryotic translation initiation factor that regulates both ribosome biogenesis and translation, which are rate-limiting factors for cell growth. The encoded protein binds 60S ribosomes and prevents their association with 40S ribosomes. This gene may play a role in oncogenesis as well. [provided by RefSeq, Aug 2015]
Uniprot Description
EIF6: eukaryotic translation initiation factor 6. Binds to the 60S ribosomal subunit and prevents its association with the 40S ribosomal subunit to form the 80S initiation complex.
Protein type: Nucleolus; Translation initiation; Translation
Cellular Component: nucleoplasm; cytoplasm; lamin filament; intermediate filament; nucleus
Molecular Function: translation initiation factor activity; ribosome binding
Biological Process: translation; ribosome biogenesis and assembly; translational initiation; mature ribosome assembly
Research Articles on EIF6
Similar Products
Product Notes
The Human EIF6 eif6 (Catalog #AAA2020780) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2020780 ELISA Kit recognizes Human EIF6. It is sometimes possible for the material contained within the vial of "Eukaryotic Translation Initiation Factor 6 (EIF6), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
