Human Eukaryotic Translation Initiation Factor 6 (EIF6) ELISA Kit | EIF6 elisa kit

Human Eukaryotic Translation Initiation Factor 6 (EIF6) ELISA Kit

Cat. Num. AAA452646

• Gene Names: EIF6; CAB; EIF3A; eIF-6; p27BBP; ITGB4BP; b(2)gcn; p27(BBP)
• Reactivity: Human
• Synonyms: Eukaryotic Translation Initiation Factor 6 (EIF6); Human Eukaryotic Translation Initiation Factor 6 (EIF6) ELISA Kit; GDNF family receptor alpha-1; GDNF receptor alpha-1; GDNFRA; GDNFR-alpha-1; GFR-alpha-1; RET ligand 1; RETL1; TGF-beta-related neurotrophic factor receptor 1; TRNR1; EIF6 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of EIF6. No significant cross-reactivity or interference between EIF6 and analogues was observed.
• Sequence Length: 245

• Samples: Tissue homogenates, cell lysates and other biological fluids.
• Assay Type: Sandwich
• Detection Range: 0.312-20ng/mL
• Sensitivity: 0.123ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level EIF6 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level EIF6 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for EIF6 elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of EIF6 in human tissue homogenates, cell lysates and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to EIF6. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to EIF6. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain EIF6, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of EIF6 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 392494084
• NCBI GeneID: 3692
• NCBI Accession #: NP_001254739.1
• NCBI GenBank Nucleotide #: NM_001267810.1
• UniProt Accession #: P56537; Q6IBN8; Q96TD5; B7ZBG9
• Molecular Weight: 23,735 Da
• NCBI Official Full Name: eukaryotic translation initiation factor 6 isoform a
• NCBI Official Synonym Full Names: eukaryotic translation initiation factor 6
• NCBI Official Symbol: EIF6
• NCBI Official Synonym Symbols: CAB; EIF3A; eIF-6; p27BBP; ITGB4BP; b(2)gcn; p27(BBP)
• NCBI Protein Information: eukaryotic translation initiation factor 6
• UniProt Protein Name: Eukaryotic translation initiation factor 6
• Protein Family: Translation initiation factor
• UniProt Gene Name: EIF6
• UniProt Synonym Gene Names: eIF-6

NCBI Description

Hemidesmosomes are structures which link the basal lamina to the intermediate filament cytoskeleton. An important functional component of hemidesmosomes is the integrin beta-4 subunit (ITGB4), a protein containing two fibronectin type III domains. The protein encoded by this gene binds to the fibronectin type III domains of ITGB4 and may help link ITGB4 to the intermediate filament cytoskeleton. The encoded protein, which is insoluble and found both in the nucleus and in the cytoplasm, can function as a translation initiation factor and prevent the association of the 40S and 60S ribosomal subunits. Multiple non-protein coding transcript variants and variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jun 2012]

Uniprot Description

Binds to the 60S ribosomal subunit and prevents its association with the 40S ribosomal subunit to form the 80S initiation complex in the cytoplasm. Behaves as a stimulatory translation initiation factor downstream insulin/growth factors. Is also involved in ribosome biogenesis. Associates with pre-60S subunits in the nucleus and is involved in its nuclear export. Cytoplasmic release of TIF6 from 60S subunits and nuclear relocalization is promoted by a RACK1 (RACK1)-dependent protein kinase C activity (PubMed:10085284, PubMed:14654845, PubMed:21536732). In tissues responsive to insulin, controls fatty acid synthesis and glycolysis by exerting translational control of adipogenic transcription factors such as CEBPB, CEBPD and ATF4 that have G/C rich or uORF in their 5'UTR. Required for ROS-dependent megakaryocyte maturation and platelets formation, controls the expression of mitochondrial respiratory chain genes involved in reactive oxygen species (ROS) synthesis (). Involved in miRNA-mediated gene silencing by the RNA-induced silencing complex (RISC). Required for both miRNA-mediated translational repression and miRNA-mediated cleavage of complementary mRNAs by RISC (PubMed:17507929). Modulates cell cycle progression and global translation of pre-B cells, its activation seems to be rate-limiting in tumorigenesis and tumor growth ().