Human Erythrocyte membrane protein band 4.2 ELISA Kit | EPB42 elisa kit

Human Erythrocyte membrane protein band 4.2 ELISA Kit

Cat. Num. AAA763258

• Reactivity: Human
• Synonyms: Erythrocyte membrane protein band 4.2; Human Erythrocyte membrane protein band 4.2 ELISA Kit; EPB42/Erythrocyte protein 4.2/P4.2/E42P; EPB42 elisa kit

• Reactivity: Human
• Sequence Length: 691

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.156-10ng/ml
• Sensitivity: 0.094ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for EPB42 elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Human EPB42antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human EPB42 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human EPB42 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration ofHuman EPB42can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 182084
• Molecular Weight: 69,455 Da
• NCBI Official Full Name: erythrocyte membrane protein band 4.2
• UniProt Protein Name: Erythrocyte membrane protein band 4.2
• Protein Family: Erythrocyte membrane protein
• UniProt Gene Name: EPB42
• UniProt Synonym Gene Names: E42P; Erythrocyte protein 4.2; P4.2
• UniProt Entry Name: EPB42_HUMAN

Uniprot Description

Epb4.2: Probably plays an important role in the regulation of erythrocyte shape and mechanical properties. Defects in EPB42 are the cause of spherocytosis type 5 (SPH5); also known as hereditary spherocytosis type 5 (HS5). Spherocytosis is a hematologic disorder leading to chronic hemolytic anemia and characterized by numerous abnormally shaped erythrocytes which are generally spheroidal. Absence of band 4.2 associated with spur or target erythrocytes has also been reported. Belongs to the transglutaminase superfamily. Transglutaminase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cytoskeletal

Chromosomal Location of Human Ortholog: 15q15-q21

Cellular Component: cortical cytoskeleton; cytoskeleton; plasma membrane

Molecular Function: ATP binding; protein binding; protein-glutamine gamma-glutamyltransferase activity; structural constituent of cytoskeleton

Biological Process: cell morphogenesis; erythrocyte maturation; hemoglobin metabolic process; iron ion homeostasis; peptide cross-linking; regulation of cell shape; spleen development

Disease: Spherocytosis, Type 5