DCBLD2 recombinant protein

DCBLD2 Protein, Human, Recombinant (His Tag)

Cat. Num. AAA8121476

• Gene Names: DCBLD2; ESDN; CLCP1
• Purity: >95% as determined by SDS-PAGE
• Synonyms: DCBLD2; DCBLD2 Protein; Human; Recombinant (His Tag); Human DCBLD2/ESDN/CLCP1 Protein (His Tag); CLCP1 Protein; ESDN Protein; discoidin; CUB and LCCL domain containing 2; DCBLD2 recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Ala482

• Species: Human
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Gln 21
• Tag: C-His
• Protein Construction: A DNA sequence encoding the human DCBLD2 (NP_563615.3)(Met1-Ala482) was expressed with a polyhistidine tag at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for DCBLD2 recombinant protein

Background: DCBLD2, also known as ESDN and CLCP1, localizes in various compartments. DCBLD2 is up-regulated in lung cancers and is regulated by transcription factor AP-2 alpha (TFAP2A), a component of activator protein-2 (AP-2) that is known to regulate IL-8 production in human lung fibroblasts and epithelial cells. DCBLD2 could be related to FEV(1)-related phenotypes in asthmatics. DCBLD2 gene is expressed at very high level. DCBLD2 is proposed to participate in processes such as intracellular receptor mediated signaling pathway, negative regulation of cell growth and so on.

References

Kobuke K. et al., 2001, J Biol Chem. 276 (36): 34105-14. Adeghi MM. et al., 2007, Am J Transplant. 7 (9): 2098-105. Chen Y. et al., 2007, Proteomics. 7 (14): 2384-97. Park TJ. et al., 2012, J Korean Med Sci. 27 (4): 343-9.

NCBI and Uniprot Product Information

• NCBI GI #: 54792129
• NCBI GeneID: 131566
• NCBI Accession #: NP_563615.3
• NCBI GenBank Nucleotide #: NM_080927.3
• UniProt Accession #: Q96PD2; Q8N6M4; Q8TDX2; B7WNL1; D3DN41
• Molecular Weight: 86,657 Da
• NCBI Official Full Name: discoidin, CUB and LCCL domain-containing protein 2
• NCBI Official Synonym Full Names: discoidin, CUB and LCCL domain containing 2
• NCBI Official Symbol: DCBLD2
• NCBI Official Synonym Symbols: ESDN; CLCP1
• NCBI Protein Information: discoidin, CUB and LCCL domain-containing protein 2; 1700055P21Rik; coagulation factor V/VIII-homology domains protein 1; CUB, LCCL and coagulation factor V/VIII-homology domains protein 1; endothelial and smooth muscle cell-derived neuropilin-like protei
• UniProt Protein Name: Discoidin, CUB and LCCL domain-containing protein 2
• Protein Family: Discoidin, CUB and LCCL domain-containing protein
• UniProt Gene Name: DCBLD2
• UniProt Synonym Gene Names: CLCP1; ESDN
• UniProt Entry Name: DCBD2_HUMAN

Uniprot Description

DCBLD2: a type-I transmembrane protein. May be involved in vascular remodeling and influence vascular smooth muscle cell proliferation. Contains a CUB domain and a coagulation factor V/VIII homology domain, similar to the structure of the neuropilins. Plays a role in cell motility. SEMA4B appears to be one of its ligands. Strongly expressed in nerve bundles. Highly expressed in testis, heart, skeletal muscle and also in cultured vascular smooth muscle cells. Increased in lung cancers during the process of tumor progression. A target of EGF signaling in the A431 cervical cancer cell-line. Two isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Cell adhesion

Chromosomal Location of Human Ortholog: 3q12.1|3

Cellular Component: cell surface; integral to plasma membrane

Molecular Function: protein binding

Biological Process: intracellular receptor-mediated signaling pathway; wound healing; negative regulation of cell growth