Porcine erythrocyte membrane protein (EMP) ELISA Kit | EMP elisa kit

Porcine erythrocyte membrane protein (EMP) ELISA Kit

Cat. Num. AAA265383

• Reactivity: Porcine
• Synonyms: erythrocyte membrane protein (EMP); Porcine erythrocyte membrane protein (EMP) ELISA Kit; EMP elisa kit

• Reactivity: Porcine
• Specificity: No cross-reaction with other factors.
• Sequence Length: 721

• Samples: Porcine Serum, Plasma Or Cell Culture Supernatant And Organizations In The Natural And Recombinant Eg-Vegf Concentration
• Assay Type: Sandwich
• Detection Range: 1000 pg/ml-15.6 pg/ml
• Sensitivity: 5 pg/ml.
• Intra-assay Precision: <= 8%
• Inter-assay Precision: <= 12%
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for EMP elisa kit

Principle of the Assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Porcine EG-VEGF monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

NCBI and Uniprot Product Information

• NCBI GI #: 951198
• Molecular Weight: 69,455 Da
• NCBI Official Full Name: erythrocyte membrane protein
• UniProt Protein Name: Erythrocyte membrane protein band 4.2
• Protein Family: Extracellular matrix protein-binding protein
• UniProt Gene Name: EPB42
• UniProt Synonym Gene Names: E42P; Erythrocyte protein 4.2; P4.2
• UniProt Entry Name: EPB42_HUMAN

Uniprot Description

Epb4.2: Probably plays an important role in the regulation of erythrocyte shape and mechanical properties. Defects in EPB42 are the cause of spherocytosis type 5 (SPH5); also known as hereditary spherocytosis type 5 (HS5). Spherocytosis is a hematologic disorder leading to chronic hemolytic anemia and characterized by numerous abnormally shaped erythrocytes which are generally spheroidal. Absence of band 4.2 associated with spur or target erythrocytes has also been reported. Belongs to the transglutaminase superfamily. Transglutaminase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cytoskeletal

Chromosomal Location of Human Ortholog: 15q15-q21

Cellular Component: cortical cytoskeleton; cytoskeleton; plasma membrane

Molecular Function: protein binding; protein-glutamine gamma-glutamyltransferase activity; structural constituent of cytoskeleton; ATP binding

Biological Process: spleen development; regulation of cell shape; iron ion homeostasis; erythrocyte maturation; hemoglobin metabolic process; cell morphogenesis; peptide cross-linking

Disease: Spherocytosis, Type 5