Human ERRFI1 (ERBB receptor feedback inhibitor 1) ELISA Kit | ERRFI1 elisa kit

Human ERRFI1 (ERBB receptor feedback inhibitor 1) ELISA Kit

Cat. Num. AAA7607015

• Gene Names: ERRFI1; MIG6; RALT; MIG-6; GENE-33
• Reactivity: Human
• Synonyms: ERRFI1 (ERBB receptor feedback inhibitor 1); Human ERRFI1 (ERBB receptor feedback inhibitor 1) ELISA Kit; ERRFI1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of ERRFI1. No significant cross-reactivity or interference between ERRFI1 and analogues was observed.
• Sequence Length: 462

• Samples: serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 0.156-10ng/ml
• Sensitivity: <0.094ng/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level ERRFI1 were tested 20 times on one plate, respectively. Intra-Assay: CV<8%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level ERRFI1 were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%
• Preparation and Storage: Store at 4 degree C for 6 months.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ERRFI1 elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- ERRFI1 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- ERRFI1 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the ERRFI1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of ERRFI1 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 10047086
• NCBI GeneID: 54206
• NCBI Accession #: NP_061821.1
• NCBI GenBank Nucleotide #: NM_018948.3
• UniProt Accession #: Q9UJM3; Q9NTG9; Q9UD05; B2RDX9
• Molecular Weight: 50,560 Da
• NCBI Official Full Name: ERBB receptor feedback inhibitor 1
• NCBI Official Synonym Full Names: ERBB receptor feedback inhibitor 1
• NCBI Official Symbol: ERRFI1
• NCBI Official Synonym Symbols: MIG6; RALT; MIG-6; GENE-33
• NCBI Protein Information: ERBB receptor feedback inhibitor 1
• UniProt Protein Name: ERBB receptor feedback inhibitor 1
• Protein Family: ERBB receptor feedback inhibitor
• UniProt Gene Name: ERRFI1
• UniProt Synonym Gene Names: MIG6; MIG-6

NCBI Description

ERRFI1 is a cytoplasmic protein whose expression is upregulated with cell growth (Wick et al., 1995 [PubMed 7641805]). It shares significant homology with the protein product of rat gene-33, which is induced during cell stress and mediates cell signaling (Makkinje et al., 2000 [PubMed 10749885]; Fiorentino et al., 2000 [PubMed 11003669]).[supplied by OMIM, Mar 2008]

Uniprot Description

Negative regulator of EGFR signaling in skin morphogenesis. Acts as a negative regulator for several EGFR family members, including ERBB2, ERBB3 and ERBB4. Inhibits EGFR catalytic activity by interfering with its dimerization. Inhibits autophosphorylation of EGFR, ERBB2 and ERBB4. Important for normal keratinocyte proliferation and differentiation. Plays a role in modulating the response to steroid hormones in the uterus. Required for normal response to progesterone in the uterus and for fertility. Mediates epithelial estrogen responses in the uterus by regulating ESR1 levels and activation. Important for regulation of endometrium cell proliferation. Important for normal prenatal and perinatal lung development ().