Rabbit anti-Human ERRFI1 Polyclonal Antibody | anti-ERRFI1 antibody

ERRFI1, ID (ERRFI1, MIG6, ERBB receptor feedback inhibitor 1, Mitogen-inducible gene 6 protein) (Biotin)

Cat. Num. AAA6296670

• Gene Names: ERRFI1; MIG6; RALT; MIG-6; GENE-33
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: ERRFI1; Polyclonal Antibody; ID (ERRFI1; MIG6; ERBB receptor feedback inhibitor 1; Mitogen-inducible gene 6 protein) (Biotin); Mitogen-inducible gene 6 protein; anti-ERRFI1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-ERRFI1 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: ERRFI1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 256-286 amino acids from the Central region of human ERRFI1.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(ERRFI1 Antibody (Center) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the ERRFI1 antibody detected the ERRFI1 protein (arrow).)

Related Product Information for anti-ERRFI1 antibody

ERRFI1 is a cytoplasmic protein whose expression is upregulated with cell growth (Wick et al., 1995 [PubMed 7641805]). It shares significant homology with the protein product of rat gene-33, which is induced during cell stress and mediates cell signaling (Makkinje et al., 2000 [PubMed 10749885]; Fiorentino et al., 2000 [PubMed 11003669]).

Product Categories/Family for anti-ERRFI1 antibody

Antibodies; Receptors

References

Frosi, Y., et al. J. Cell Biol. 189(3):557-571(2010). Ying, H., et al. Proc. Natl. Acad. Sci. U.S.A. 107(15):6912-6917(2010). Dubois, P.C., et al. Nat. Genet. 42(4):295-302(2010). Reschke, M., et al. Hepatology 51(4):1383-1390(2010). Nagashima, T., et al. FEBS J. 276(18):5239-5251(2009).

NCBI and Uniprot Product Information

• NCBI GI #: 10047086
• NCBI GeneID: 54206
• NCBI Accession #: NP_061821.1
• NCBI GenBank Nucleotide #: NM_018948.3
• UniProt Accession #: Q9UJM3; Q9NTG9; Q9UD05; B2RDX9
• Molecular Weight: 50,560 Da
• NCBI Official Full Name: ERBB receptor feedback inhibitor 1
• NCBI Official Synonym Full Names: ERBB receptor feedback inhibitor 1
• NCBI Official Symbol: ERRFI1
• NCBI Official Synonym Symbols: MIG6; RALT; MIG-6; GENE-33
• NCBI Protein Information: ERBB receptor feedback inhibitor 1
• UniProt Protein Name: ERBB receptor feedback inhibitor 1
• Protein Family: ERBB receptor feedback inhibitor
• UniProt Gene Name: ERRFI1
• UniProt Synonym Gene Names: MIG6; MIG-6
• UniProt Entry Name: ERRFI_HUMAN

NCBI Description

ERRFI1 is a cytoplasmic protein whose expression is upregulated with cell growth (Wick et al., 1995 [PubMed 7641805]). It shares significant homology with the protein product of rat gene-33, which is induced during cell stress and mediates cell signaling (Makkinje et al., 2000 [PubMed 10749885]; Fiorentino et al., 2000 [PubMed 11003669]).[supplied by OMIM, Mar 2008]

Uniprot Description

MIG-6: Negative regulator of EGFR signaling in skin morphogenesis. Acts as a negative regulator for several EGFR family members, including ERBB2, ERBB3 and ERBB4. Inhibits EGFR catalytic activity by interfering with its dimerization. Inhibits autophosphorylation of EGFR, ERBB2 and ERBB4. Important for normal keratinocyte proliferation and differentiation. Plays a role in modulating the response to steroid hormones in the uterus. Required for normal response to progesterone in the uterus and for fertility. Mediates epithelial estrogen responses in the uterus by regulating ESR1 levels and activation. Important for regulation of endometrium cell proliferation. Important for normal prenatal and perinatal lung development. Interacts with ERBB2. Interacts with EGFR. Levels are very low in quiescent cells. Up-regulated by mitogens. Belongs to the MIG6 family.

Protein type: Tumor suppressor; Inhibitor

Chromosomal Location of Human Ortholog: 1p36

Cellular Component: extrinsic to internal side of plasma membrane; cytoplasm; nucleus

Molecular Function: protein binding; protein kinase binding

Biological Process: negative regulation of epidermal growth factor receptor activity; skin morphogenesis; response to stress; negative regulation of protein amino acid autophosphorylation; regulation of keratinocyte differentiation; alveolus development