Human Endothelial Nitric Oxide Synthase 3 (eNOS-3) ELISA Kit | eNOS-3 elisa kit

Human Endothelial Nitric Oxide Synthase 3 (eNOS-3) ELISA Kit

Cat. Num. AAA265475

• Gene Names: Nos3; eNos
• Reactivity: Human
• Synonyms: Endothelial Nitric Oxide Synthase 3 (eNOS-3); Human Endothelial Nitric Oxide Synthase 3 (eNOS-3) ELISA Kit; eNOS-3 elisa kit

• Reactivity: Human
• Sequence Length: 306

• Samples: Human serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant eNOS-3 concentration
• Assay Type: Sandwich
• Detection Range: 100 U/ml-1.56 U/ml
• Sensitivity: 0.5 U/ml.
• Intra-assay Precision: <= 8%
• Inter-assay Precision: <= 12%
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for eNOS-3 elisa kit

Principle of the Assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is human eNOS-3 monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

NCBI and Uniprot Product Information

• NCBI GI #: 7259222
• NCBI GeneID: 24600
• UniProt Accession #: Q9JJY7; Q62600
• Molecular Weight: 33,804 Da
• NCBI Official Full Name: endothelial nitric oxide synthase 3, partial
• NCBI Official Synonym Full Names: nitric oxide synthase 3, endothelial cell
• NCBI Official Symbol: Nos3
• NCBI Official Synonym Symbols: eNos
• NCBI Protein Information: nitric oxide synthase, endothelial; cNOS; EC-NOS; NOSIII; NOS type III; endothelial NOS; constitutive NOS; endothelial nitric oxide synthase 3
• UniProt Protein Name: Endothelial nitric oxide synthase 3
• UniProt Gene Name: nos3
• UniProt Entry Name: Q9JJY7_RAT

NCBI Description

enzyme that synthesizes Nitric oxide from L-arginine [RGD, Feb 2006]

Uniprot Description

eNOS: endothelial constitutive nitric oxide synthase. Synthesizes nitric oxide (NO) from arginine and oxygen, which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets. Induced in humans by caloric restriction. Stimulated by calcium/calmodulin. Inhibited by NOSIP and NOSTRIN.

Protein type: Oxidoreductase; EC 1.14.13.39; Amino Acid Metabolism - arginine and proline

Cellular Component: Golgi apparatus; cytoskeleton; mitochondrion; apical part of cell; cytoplasm; nucleolus; plasma membrane; vesicle membrane; caveola; cytosol; nucleus; sarcolemma; lipid raft

Molecular Function: actin monomer binding; FAD binding; nitric-oxide synthase activity; beta-catenin binding; Hsp90 protein binding; actin binding; calmodulin binding; protein binding; NADPH-hemoprotein reductase activity; FMN binding; cadherin binding; iron ion binding; heme binding; nitric-oxide synthase binding; NADP binding

Biological Process: response to metal ion; response to peptide hormone stimulus; regulation of systemic arterial blood pressure by endothelin; positive regulation of apoptosis; negative regulation of smooth muscle cell proliferation; negative regulation of potassium ion transport; response to lipopolysaccharide; negative regulation of muscle hyperplasia; female pregnancy; signal transduction; positive regulation of vasodilation; response to organic cyclic substance; response to estradiol stimulus; negative regulation of cell proliferation; negative regulation of hydrolase activity; regulation of the force of heart contraction by chemical signal; lipopolysaccharide-mediated signaling pathway; response to lipoprotein stimulus; angiogenesis; response to axon injury; negative regulation of blood pressure; regulation of blood vessel size; nitric oxide biosynthetic process; response to fructose stimulus; response to corticosterone stimulus; aging; response to drug; negative regulation of calcium ion transport; in utero embryonic development; positive regulation of guanylate cyclase activity; regulation of sodium ion transport; ovulation from ovarian follicle; response to amino acid stimulus; positive regulation of angiogenesis; response to ethanol; response to hyperoxia; response to mechanical stimulus; retina development in camera-type eye; response to cytokine stimulus; response to lead ion; response to hypoxia; arginine catabolic process; endothelial cell migration; blood vessel remodeling; response to activity; nitric oxide mediated signal transduction; lung development; smooth muscle hyperplasia