Bovine nitric oxide synthase (NOS) ELISA Kit | NOS elisa kit

Bovine nitric oxide synthase(NOS) ELISA KIT

Cat. Num. AAA2609572

• Gene Names: NOS3; eNOS; ECNOS
• Reactivity: Bovine
• Synonyms: nitric oxide synthase (NOS); Bovine nitric oxide synthase(NOS) ELISA KIT; NOS elisa kit

• Reactivity: Bovine
• Specificity: No cross-reaction with other factors.
• Sequence Length: 118

• Samples: Serum, plasma or cell culture supernatant and organizations in the natural and recombinant NOS concentration
• Assay Type: Sandwich
• Detection Range: 20 ng/ml-0.312 ng/ml
• Sensitivity: 0.06 ng/ml.
• Intra-assay Precision: ? 8%
• Inter-assay Precision: ? 12%
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for NOS elisa kit

Principle of the Assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Bovine NOS monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

NCBI and Uniprot Product Information

• NCBI GI #: 4262036
• NCBI GeneID: 4846
• Molecular Weight: 67,876 Da
• NCBI Official Full Name: nitric oxide synthase, partial
• NCBI Official Synonym Full Names: nitric oxide synthase 3
• NCBI Official Symbol: NOS3
• NCBI Official Synonym Symbols: eNOS; ECNOS
• NCBI Protein Information: nitric oxide synthase, endothelial
• UniProt Protein Name: Nitric oxide synthase, endothelial
• Protein Family: Nitric oxide synthase
• UniProt Gene Name: NOS3
• UniProt Synonym Gene Names: cNOS; eNOS; NOSIII
• UniProt Entry Name: NOS3_HUMAN

NCBI Description

Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. Nitric oxide is synthesized from L-arginine by nitric oxide synthases. Variations in this gene are associated with susceptibility to coronary spasm. Alternative splicing and the use of alternative promoters results in multiple transcript variants. [provided by RefSeq, Oct 2016]

Uniprot Description

eNOS: endothelial constitutive nitric oxide synthase. Synthesizes nitric oxide (NO) from arginine and oxygen, which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets. Induced in humans by caloric restriction. Stimulated by calcium/calmodulin. Inhibited by NOSIP and NOSTRIN.

Protein type: Amino Acid Metabolism - arginine and proline; EC 1.14.13.39; Oxidoreductase

Chromosomal Location of Human Ortholog: 7q36

Cellular Component: caveola; cytoplasm; cytosol; Golgi membrane; nucleus; plasma membrane

Molecular Function: actin monomer binding; heme binding; nitric-oxide synthase activity; protein binding

Biological Process: arginine catabolic process; blood vessel remodeling; cell redox homeostasis; endothelial cell migration; mitochondrion organization and biogenesis; negative regulation of blood pressure; negative regulation of cell proliferation; negative regulation of muscle hyperplasia; nitric oxide biosynthetic process; nitric oxide mediated signal transduction; positive regulation of angiogenesis; positive regulation of guanylate cyclase activity; positive regulation of vasodilation; regulation of blood vessel size; regulation of nitric-oxide synthase activity; regulation of systemic arterial blood pressure by endothelin; removal of superoxide radicals; smooth muscle hyperplasia

Disease: Alzheimer Disease; Hypertension, Essential; Preeclampsia/eclampsia 1; Stroke, Ischemic