Human Elastin Microfibril Interface Located Protein 1 (EMILIN1) ELISA Kit | EMILIN1 elisa kit

Human Elastin Microfibril Interface Located Protein 1 (EMILIN1) ELISA Kit

Cat. Num. AAA2701774

• Gene Names: EMILIN1; EMI; gp115; EMILIN
• Reactivity: Human
• Synonyms: Elastin Microfibril Interface Located Protein 1 (EMILIN1); Human Elastin Microfibril Interface Located Protein 1 (EMILIN1) ELISA Kit; EMI; gp115; Elastin Microfibril Interfacer 1; EMILIN1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Elastin Microfibril Interface Located Protein 1 (EMILIN1).; No significant cross-reactivity or interference between Elastin Microfibril Interface Located Protein 1 (EMILIN1) and analogues was observed.
• Sequence Length: 1016

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma, Tissue Homogenates and Other Biological Fluids
• Detection Range: 1.56-100ng/mL
• Sensitivity: < 0.56ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Elastin Microfibril Interface Located Protein 1 (EMILIN1) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Elastin Microfibril Interface Located Protein 1 (EMILIN1) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for EMILIN1 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Elastin Microfibril Interface Located Protein 1 (EMILIN1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Elastin Microfibril Interface Located Protein 1 (EMILIN1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Elastin Microfibril Interface Located Protein 1 (EMILIN1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Elastin Microfibril Interface Located Protein 1 (EMILIN1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 5901944
• NCBI GeneID: 11117
• NCBI Accession #: NP_008977.1
• NCBI GenBank Nucleotide #: NM_007046.3
• UniProt Accession #: Q9Y6C2; Q53SY9; Q96G58; Q96IH6; Q9UG76; A0A0C4DFX3; A5PL03; H0Y7A0
• Molecular Weight: 35,928 Da
• NCBI Official Full Name: EMILIN-1
• NCBI Official Synonym Full Names: elastin microfibril interfacer 1
• NCBI Official Symbol: EMILIN1
• NCBI Official Synonym Symbols: EMI; gp115; EMILIN
• NCBI Protein Information: EMILIN-1
• UniProt Protein Name: EMILIN-1
• Protein Family: EMILIN
• UniProt Gene Name: EMILIN1
• UniProt Synonym Gene Names: EMI; Elastin microfibril interfacer 1

NCBI Description

This gene encodes an extracellular matrix glycoprotein that is characterized by an N-terminal microfibril interface domain, a coiled-coiled alpha-helical domain, a collagenous domain and a C-terminal globular C1q domain. The encoded protein associates with elastic fibers at the interface between elastin and microfibrils and may play a role in the development of elastic tissues including large blood vessels, dermis, heart and lung. [provided by RefSeq, Sep 2009]

Uniprot Description

May be responsible for anchoring smooth muscle cells to elastic fibers, and may be involved not only in the formation of the elastic fiber, but also in the processes that regulate vessel assembly. Has cell adhesive capacity.